Application of carbon quantum dots/cuprous oxide (CQDs/Cu2O) compound to preparation of cancer treating medicines

A technology of cuprous oxide and carbon quantum dots is applied in the application field of medicine, which can solve the problem of unreported anti-tumor activity, and achieve the effects of inhibiting cell migration, inhibiting cell proliferation and high toxicity.

Pending Publication Date: 2018-01-12
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, about carbon quantum dots / cuprous oxide (CQDs / Cu 2 O) The research on the anti-tumor activity of the complex has not been reported

Method used

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  • Application of carbon quantum dots/cuprous oxide (CQDs/Cu2O) compound to preparation of cancer treating medicines
  • Application of carbon quantum dots/cuprous oxide (CQDs/Cu2O) compound to preparation of cancer treating medicines
  • Application of carbon quantum dots/cuprous oxide (CQDs/Cu2O) compound to preparation of cancer treating medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 CQDs / Cu 2 Preparation of O and CQDs

[0038] Weigh 6g NaOH and 3.74g copper sulfate and dissolve them in 150mL deionized water respectively, add the NaOH solution to 0.1mol·L -1 In the copper sulfate solution, a flocculent suspension is formed, and ultrasonic (150W, 40KH) is used for 15 minutes. Weigh 1 g of polyvinylpyrrolidone, dissolve it in deionized water, and add it to the above mixture. Then weigh 18g of glucose and dissolve it in 100mL (1mol·L -1 ) deionized water was added to the mixture, and ultrasonic treatment was performed for 60 minutes. After standing for 16 hours, it was washed three times with deionized water and absolute ethanol, and dried in an oven at 60°C for 6 hours to obtain CQDs / Cu 2 O composite materials.

[0039] Weigh 18g of glucose and dissolve it in deionized water, add 1mol·L -1 The NaOH solution was ultrasonically treated for 5 h to adjust the pH to 7.0, and CQDs were obtained after purification with ethanol and magnesium s...

Embodiment 2

[0046] This example studies the CQDs / Cu in depth 2 Effects of O on the migration, apoptosis, cell cycle, expression of F-actin, matrix metalloproteinase MMP-2, and angiogenesis of SKOV3 cells.

[0047] cell culture

[0048] Cells use low-glucose medium (DMEM) containing 10% fetal bovine serum; BABL-3T3 cells use high-glucose medium containing 15% fetal bovine serum; HUVEC cells are extracted from human umbilical cord with 0.01% collagenase; %BI culture medium. All the cells were cultured in a 37°C, 5% carbon dioxide incubator, and subcultured when the cell confluency reached more than 80%.

[0049] Cell digestion, passaging

[0050] When the cell growth reaches more than 80%, remove the original culture medium in the culture flask, wash once with preheated PBS buffer, digest with preheated 0.25% trypsin for 3-5min, add 10mL DMEM culture medium (containing fetal bovine serum) ), centrifuge for 5min, remove the cells, add 10mL of fresh culture medium, and put them into the i...

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Abstract

The invention discloses application of a carbon quantum dots/cuprous oxide (CQDs/Cu2O) compound to preparation of cancer treating medicines. Through in-vitro cell experiments, the anticancer activityof the CQDs/Cu2O is explored; after the CQDs/Cu2O acts on five types of cancer cells, namely HeLa, A549, HT-29, SKOV3 and BABL-3T3, for 72h, the IC50 value is 1.25-18.9[mu]g.mL<-1>, the cancer cell proliferation inhibiting effect is higher than the normal cell proliferation inhibiting effect, and the toxicity and side effects on normal cells are relatively low; when the concentration is 12.5[mu]g.mL<-1>, the cancer cell inhibiting rate is higher than 50%; the CQDs/Cu2O has the strongest SKOV3 cell inhibiting effect, and the IC50 value is 12.5[mu]g.mL<-1>; the CQDs/Cu2O is the most sensitive toSKOV3 cells.

Description

technical field [0001] The invention belongs to the technical field of anticancer materials, in particular to carbon quantum dots / cuprous oxide (CQDs / Cu 2 O) Application of the complex in the preparation of a medicament for treating cancer. Background technique [0002] With the improvement of human living standards and the aggravation of environmental pollution, the morbidity and mortality of cancer continue to increase, especially ovarian cancer, which is the second most lethal gynecological malignancy in the world. Tumor treatment methods include surgical treatment, radiotherapy and drug treatment. Currently, the conventional clinical treatment plan is to perform drug treatment on the basis of cytoreductive surgery. Because anti-tumor chemotherapy drugs often have serious adverse reactions and drug resistance problems, which seriously affect the effect of chemotherapy and the prognosis of patients, their application is greatly limited. Conventional anti-cancer treatment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61P35/00A61P35/04A61K33/34
Inventor 李斌陈道梅王家强聂敏芳
Owner YUNNAN UNIV
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