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Human lectin/human lectin-like chip for glycosylation detection and preparation method thereof

A technology for detecting chips and lectins, which is applied in the field of biological sciences and can solve problems such as hindering the research process, low recombinant expression, and low separation and purification efficiency

Active Publication Date: 2021-07-06
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Because most human lectins or human-like lectins are membrane proteins, they have the disadvantages of low recombinant expression, low separation and purification efficiency, and easy inactivation, which hinder their research progress
No human lectin chips have been reported so far

Method used

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  • Human lectin/human lectin-like chip for glycosylation detection and preparation method thereof
  • Human lectin/human lectin-like chip for glycosylation detection and preparation method thereof
  • Human lectin/human lectin-like chip for glycosylation detection and preparation method thereof

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Embodiment Construction

[0035] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0036] This example provides a human lectin / human lectin-like protein chip and an activity verification method. For the chip construction process, see figure 1 . The following is the detailed process and parameters of the whole process:

[0037] 1. Obtain the yeast expression library of human lectin and human lectin-like protein

[0038] The entry clone library of 60 human lectins and human-like lectins comes from Professor Zhu Heng of Johns Hopkins University.

[0039]After the sequencing is...

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Abstract

The invention discloses a human lectin / human lectin-like chip for glycosylation detection in the field of biological sciences and a preparation method, which is constructed based on recombinant human lectin / human lectin-like protein. The recombinant proteins are 60 kinds of human lectin / human lectin-like proteins capable of recognizing and binding different sugar groups. Compared with the traditional plant lectin chip, the human lectin / human lectin chip has more specific recognition ability and stronger binding ability to complex glycoforms of higher vertebrate samples. Human lectin / human lectin-like chips provide a new technology platform for glycomics research and can be widely used in glycosylation research of humans or other mammals.

Description

technical field [0001] The invention relates to the field of biological sciences, in particular to a human lectin / human lectin-like chip for glycosylation detection and a preparation method thereof. Structure-specific recognition and binding to analyze the glycosylation status and glycosylation level changes of biological samples. Background technique [0002] Glycosylation is the most important post-translational modification in organisms, and more than 50% of proteins have glycosylation modification. Glycosylation plays a key role in protein folding, cell growth, division, and differentiation. It can act as a receptor to recognize extracellular signals, and can also activate immune responses in organisms, etc. Some physiological and pathological processes are also closely related to glycosylation. For example, the sugar on the surface of sperm cells can affect the motility and fertilization ability of sperm cells, and the increase of sialic acid on the surface of cancer c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C40B40/10G01N33/68
Inventor 陶生策孙洋洋程莉周树敏郭书娟
Owner SHANGHAI JIAOTONG UNIV
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