Preparation method of rumen-derived microbial fermentation feed rich in volatile fatty acid calcium
A microbial fermentation and rumen microorganism technology, applied in the field of enzyme preparations, microorganisms, and feed, can solve the problems of irritation, difficult drying, and high drying costs, and achieve the effect of increasing feed intake and promoting digestion.
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Embodiment 1
[0059] A method for preparing rumen-derived microbial fermented feed rich in volatile fatty acid calcium, comprising the following steps:
[0060] ①. Preparation of rumen microbial strain liquid:
[0061] Gather 20kg (195L) of the rumen liquid of 5 healthy dairy cows to obtain 200kg of the rumen microbial strain liquid;
[0062] ②. Preparation of rumen microbial fermentation liquid:
[0063] Grind 50kg of soybean meal, 150kg of corn and 400kg of wheat straw, add 1400kg of water to adjust the slurry, and then pump it into the fermenter; add steam to raise the temperature to 39°C and continue to pass in CO for 20 minutes 2 After excluding the oxygen in the feed slurry, add 20kg of trace element nutrient buffer and 200kg of rumen microbial strain solution, and strictly anaerobic fermentation under the condition of continuous flow of calcium hydroxide or calcium carbonate fine powder for 72 hours, to obtain 2200kg of rumen microbial fermentation broth.
[0064] ③. Preparation o...
Embodiment 2
[0075] A method for preparing rumen-derived microbial fermented feed rich in volatile fatty acid calcium, comprising the following steps:
[0076] ①. Preparation of rumen microbial strain liquid:
[0077] Gather 5 kg (4.9 L) of the rumen liquid of 5 healthy goats to obtain 50 kg of the rumen microbial strain liquid;
[0078] ②. Preparation of rumen microbial fermentation liquid:
[0079] Grind 10kg of soybean meal, 50kg of corn and 100kg of wheat straw, add 340kg of water to adjust the slurry, and then pump it into the fermenter; add steam to raise the temperature to 39°C and continue to pass in CO for 10 minutes 2 After excluding the oxygen in the feed slurry, add 3kg of trace element nutrient buffer and 50kg of rumen microbial seed liquid, and strictly anaerobically ferment for 96 hours under the condition of continuous flow of calcium hydroxide or calcium carbonate fine powder, and obtain 550kg of rumen microbial fermentation broth.
[0080] ③. Preparation of rumen micro...
Embodiment 3
[0091] ①. Preparation of rumen microbial strain liquid:
[0092] Gather 30kg (195L) of the rumen liquid of 5 healthy beef cattle to obtain 300kg of the rumen microbial strain liquid;
[0093] ②. Preparation of rumen microbial fermentation liquid:
[0094] Grind 100kg cottonseed meal, 100kg corn, 100kg barley, 700kg wheat straw, add 2500kg water to adjust the slurry, and then pump it into the fermenter; add steam to raise the temperature to 39°C and continue to pass in CO for 20 minutes 2 After excluding the oxygen in the feed slurry, add 50kg of trace element nutrient buffer and 300kg of rumen microbial strain solution, and strictly anaerobic fermentation under the condition of continuous flow of calcium hydroxide or calcium carbonate fine powder for 72 hours, to obtain 4300kg of rumen microbial fermentation broth.
[0095] ③. Preparation of rumen microbial fermentation broth solids wet base and liquid nutrient solution:
[0096] Through solid-liquid separation equipment, 4...
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