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Cordyceps sinensis host hepialus armoricanus egg shell softening and decoloring method and softening and decoloing agent

A technology of Cordyceps sinensis host and bat moth, which is applied in animal husbandry and other fields to achieve the effect of cheap ingredients and simple operation

Active Publication Date: 2017-12-29
DONGGUAN HEC CORDYCEPS R&D CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The eggshell of the bat moth is black and shiny, with a hard texture, which is an important barrier against unfavorable environments to protect the embryonic development. There are no reports on the digestion, softening and decolorization of the eggshells of the bat moth.

Method used

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  • Cordyceps sinensis host hepialus armoricanus egg shell softening and decoloring method and softening and decoloing agent
  • Cordyceps sinensis host hepialus armoricanus egg shell softening and decoloring method and softening and decoloing agent
  • Cordyceps sinensis host hepialus armoricanus egg shell softening and decoloring method and softening and decoloing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 softening decolorization experiment

[0022] Put 1.5-2 grams of chlorine dioxide powder (chlorine dioxide content 10%) into 100mL of 50% ethanol aqueous solution (prepared and used now, absolute ethanol and water 1:1), quickly put the bat moth eggs to be tested After adding the above solution, the eggs gradually degenerate from black to brown and finally translucent light yellow. After 15-30 minutes, the softening and decolorization of egg shells is completed.

[0023] The present invention also uses different softeners and treatment methods to treat bat moth egg shells, and finds that the existing silkworm egg shell treatment methods are not suitable for bat moth eggs.

[0024] Table 1: Comparison of the effects of different softeners on the soft shell of bat moth eggs:

[0025]

[0026]

[0027] The inventor of the present invention has not obtained the softening and decolorizing method of bat moth ovum by researching various softening and decolori...

Embodiment 2

[0028] Embodiment 2 tissue section research

[0029] Get 1 gram of chlorine dioxide powder and dissolve in 60mL ethanol aqueous solution, ethanol: water=2:1, take the bat moth eggs in the early and middle stages of development and put them into the above solution, the eggs gradually change color, after 30min the eggs become light yellow, decolorized and softened After completion, the tissues were sectioned after rinsing three times with 0.9% saline.

[0030] Tissue sectioning method: the eggs were fixed with Carnoy`s Fluid for 24 hours, and then dehydrated, permeated and embedded in paraffin according to the conventional paraffin section method. The tangential direction was longitudinal section and the section thickness was 4.5 μM, patch (at a temperature of 42°C) and dry for more than 1.5 hours (the oven temperature is set at 55°C), then stained with hematoxylin-eosin counterstaining method, sealed with neutral gum, and placed in a Leica DM2500 upright microscope Observe and...

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Abstract

The invention belongs to the technical field of biology, and provides a cordyceps sinensis host hepialus armoricanus egg shell softening and decoloring method. The method is characterized in that hepialus armoricanus eggs are soaked in chlorine dioxide solution with the concentration of 0.1-0.2% to be treated for 10-60 min. The invention further provides a cordyceps sinensis host hepialus armoricanus egg shell softening and decoloring agent. The method is easy to operate and controllable, the softening and decoloring agent is low in component, green and environmentally friendly, existing technologies in the egg shell softening and decoloring field particular the hepialus armoricanus egg shell softening and decoloring technology are completely updated, and possibility is provided for further research of the hepialus armoricanus eggs.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for softening and decolorizing eggshells of the host bat moth of Cordyceps sinensis and a softening and decolorizing agent. Background technique [0002] Cordyceps sinensis is a traditional precious Chinese medicinal material in Chinese history. It is a bat moth larva parasitized in alpine meadow soil by the Cordyceps sinensis fungus of the genus Ergot in the family Ergotaceae, which makes the body of the larva ossified. The long rod-shaped sub-seat is formed at the end, that is, the complex composed of the fruiting body of Cordyceps sinensis and the sclerotium of zombie (larvae corpse). [0003] As the host of Cordyceps sinensis, bat moth larvae are extremely important for its research. The study of embryonic development is the foundation of life for eukaryotes, and the eggshell treatment technology is the premise for the developmental research of bat moth eggs, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 李文佳李全平张宗耀吕延华贺媛
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD
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