Novel quick cell stain and application thereof

A dyeing agent and cell technology, applied in the field of cell staining, can solve the problems of complex dye solution components, narrow application range, fading, etc., and achieve the effect of simple and fast dyeing operation, wide application range and stable dyeing

Active Publication Date: 2017-12-22
NAT INST FOR FOOD & DRUG CONTROL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The staining method provided by the prior art has many steps and takes a long time, and most of them need to go through a series of procedures such as cell fixation. In order to observe the cell morphology in time and quickly, the rapid staining method is often used, but the current rapid staining method generally has the following defects:
[0003] (1) Most of the reagents used in fast stains have potential pollution or harm to the environment or human body
[0004] (2) The composition of the dye solution is complex and difficult to prepare. Most of them need to be prepared and used immediately, and cannot be stored for a long time
[0005] (3) The process of using the staining agent is cumbersome, the cells need to be fixed, and the staining process takes a long time
[0006] (4) The scope of application is narrow, and generally only has fast staining effect on specific types of cells
[0007] (5) The staining time is short, and after rapid staining, it will fade in a short time, which cannot meet the needs of long-term real-time observation of living cells

Method used

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  • Novel quick cell stain and application thereof
  • Novel quick cell stain and application thereof
  • Novel quick cell stain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This embodiment provides a kind of rapid dyeing agent and its application, and described rapid dyeing agent is prepared as follows:

[0059] With 225mg toluidine blue (mass percentage 10%) and 2025mgNa 2 SO 4 (90% by mass) was dissolved in distilled water, and after the solute was completely dissolved, the volume was adjusted to 100 ml with distilled water and left to stand overnight, which was the fast dyeing agent of the present embodiment—toluidine blue fast dyeing solution. The present embodiment tests the dyeing effect of gained toluidine blue fast dyeing solution as follows:

[0060] (1) Staining of cells on microcarriers

[0061] B-16 melanoma cells were inoculated on microcarriers, and culture medium was added. Before use, take a drop of the microcarrier cell suspension containing B-16 melanoma cells and the obtained toluidine blue fast staining solution from the microcarrier culture system, put one drop each on the glass slide, mix well, stain for 0.5-2min, ...

Embodiment 2

[0077] This embodiment provides a kind of rapid dyeing agent and its application, and described rapid dyeing agent is prepared as follows:

[0078] With 50mg toluidine blue (mass percentage 5%) and 950mgNa 2 SO 4 (mass percentage 95%) was dissolved in distilled water, after the solute was completely dissolved, the volume was adjusted to 100ml with distilled water, and left to stand overnight, it was the fast dyeing agent of the present embodiment—toluidine blue fast dyeing solution.

[0079] (1) Staining of cells on microcarriers

[0080] B-16 melanoma cells were inoculated on microcarriers, and culture medium was added. Before use, take a drop of the microcarrier cell suspension containing B-16 melanoma cells and the obtained toluidine blue fast staining solution from the microcarrier culture system, put one drop each on the glass slide, mix well, stain for 0.5-2min, and observe under the microscope at the same time In case of staining, seal the slides immediately. The te...

Embodiment 3

[0096] This embodiment provides a kind of rapid dyeing agent and its application, and described rapid dyeing agent is prepared as follows:

[0097] With 400mg toluidine blue (mass percentage 14%) and 2457mgNa 2 SO4 (mass percentage 86%) was dissolved in distilled water. After the solute was completely dissolved, the volume was adjusted to 100ml with distilled water, and left to stand overnight, which was the fast dyeing agent of the present embodiment—toluidine blue fast dyeing solution.

[0098] (1) Staining of cells on microcarriers

[0099] B-16 melanoma cells were inoculated on microcarriers, and culture medium was added. Before use, take a drop of the microcarrier cell suspension containing B-16 melanoma cells and the obtained toluidine blue fast staining solution from the microcarrier culture system, put one drop each on the glass slide, mix well, stain for 0.5-2min, and observe under the microscope at the same time In case of staining, seal the slides immediately. Th...

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Abstract

The invention provides a novel quick cell stain and application thereof. The cell stain can be an aqueous solution, and comprises solute toluidine blue and sodium sulfate. The method by applying the cell stain comprises the steps of directly contacting the cell stain with a to-be-stained cell or tissue for staining so as to complete cell staining. The cell stain has the advantages of quick staining and fading prevention of stained cells. Furthermore, the cell stain has the advantages of safety, environmental friendliness, simple composition, small dosage, low price, convenience in preparation, long storage period and the like. When the cell stain is used for staining a cell attached on a microcarrier, the staining effect of the cell stain is superior to that of conventional stain, and is represented as clear cell shading, but non-stained microcarrier. The cell stain is quicker than existing mast cell stain, can be used for staining living cells, and is more sensitive and clearer to particle staining.

Description

technical field [0001] The invention relates to a cell staining agent and its application, in particular to a fast cell staining agent and its application, and belongs to the field of cell staining. Background technique [0002] In basic research, it is often necessary to observe the details of cell morphology in experiments. The fine structure of cells can be displayed in different colors through staining, so that the cell images can be clearly presented under the light microscope. The staining method provided by the prior art has many steps and takes a long time, and most of them need to go through a series of procedures such as cell fixation. In order to observe the cell morphology in time and quickly, the rapid staining method is often used, but the current rapid staining method generally has the following defects: [0003] (1) Most of the reagents used in the fast dyes have potential pollution or harm to the environment or the human body. [0004] (2) The components of...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5005
Inventor 李波赵天德刘轩房青徐波向青张露勇刘婷刘师卜
Owner NAT INST FOR FOOD & DRUG CONTROL
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