Preparation method of uric acid artificial antigen

An artificial antigen, uric acid technology, applied in chemical instruments and methods, animal/human proteins, serum albumin, etc., can solve the problems of expensive instruments, limited universal application, and inability to achieve high-throughput detection requirements, and achieve good results. Immunity effect, easy to operate effect

Inactive Publication Date: 2017-12-22
杨蕾
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Technical problems to be solved: The existing uric acid detection methods are mainly instrument-based detection methods, which require expensive instruments, complicated sample pretreatment processes, and professional operators, which limit this type of method The universal application cannot meet the high-throughput detection requirements on the spot

Method used

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  • Preparation method of uric acid artificial antigen
  • Preparation method of uric acid artificial antigen
  • Preparation method of uric acid artificial antigen

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Embodiment 1

[0024] A preparation method of uric acid artificial antigen, comprising the steps of:

[0025] (1) Modification of uric acid hapten

[0026] Weigh 20 mg of uric acid into a 100 mL round bottom flask, add 10 mL of aqueous solution, adjust the pH of the solution to 9.0 with NaOH solution to dissolve the uric acid, then add 25 mg of uric acid, and finally add 3 mL of hydrazine monohydrate solution with a purity of 99%. , put the reaction system on a water bath heating magnetic stirrer, carry out condensation and reflux reaction at 100°C for 7 hours, add 300 μL saturated sodium carbonate solution to terminate the reaction, and naturally cool the reaction system to room temperature; Three extractions were carried out, and the amount of trichloroacetic acid used each time was 15 mL. The organic phase of trichloroacetic acid was collected, and the trichloroacetic acid was spin-dried at 50° C. with a rotary evaporator to obtain the modified uric acid hapten.

[0027] (2) Synthesis of...

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Abstract

The invention relates to a preparation method of uric acid artificial antigen, and belongs to the technical field of biology and chemical engineering. The preparation method comprises the following steps of modifying of uric acid semi-antigen, and synthesizing of uric acid complete antigen, and is characterized in that firstly, the imino groups which can easily generate reaction on uric acid molecules are used as reaction groups, and generate acylation reaction with carboxyl groups on aminopropionic acid molecules, so that the modified uric acid semi-antigen contains reaction amino, and the modified amino is coupled with carrier protein. The preparation method has the advantages that the operation is simple and convenient; the antibody immunized by the prepared antigen has high sensitivity on the detection of uric acid, and the detection requirement of the uric acid is met; the important study and practical value are provided for the preparation of the uric acid antibody.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of uric acid artificial antigen. Background technique [0002] Uric acid is the final product of purine metabolism in the body. There are two main sources of uric acid in the human body. One is exogenous uric acid from the decomposition and absorption of nucleotides in food, accounting for about 20%, and the other is the catabolism of nucleotides in the human body. The endogenous uric acid produced accounts for about 80%, and uric acid is maintained at a normal level in the body through absorption and excretion. Abnormal purine metabolism in the body and increased purine in food will lead to excessive production of uric acid. Abnormal uric acid in the human body is a reflection of human diseases, mainly reflecting some diseases related to purine metabolism, such as gout and Lesch-Nyhan syndrome Wait. In recent years, research on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765
CPCC07K14/765C07K19/00
Inventor 杨蕾
Owner 杨蕾
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