Method for extracting papain by using double aqueous phase system
A two-phase system, papain technology, applied in the direction of hydrolytic enzymes, biochemical equipment and methods, enzymes, etc., can solve the problems of unstable enzyme activity and cumbersome process, and achieve simple experimental conditions, good chemical stability, and easy Magnified effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] (1) Add 2g of polyethylene glycol 400 to a 10ml centrifuge tube, and then add 4g of pre-configured NaH with a mass concentration of 60wt%. 2 PO 4 -2H 2 O solution, followed by the addition of 1g of [C with a mass concentration of 40wt% 2 mim]Cl solution, followed by the addition of 1ml of pre-prepared papain solution with a mass concentration of 10mg / ml, and finally added to 10g with distilled water.
[0021] In order to test the accuracy of the data, each sample was equipped with three parallel experiments.
[0022] (2) After the preparation of the two-phase aqueous system is completed, place it on a vortex shaker for 5 minutes to ensure uniform mixing of the entire system, and then put it in a constant temperature water bath at 25°C and let it stand for 24 hours. After the phase separation was completed, the upper and lower phase solutions were taken respectively to measure the concentration of papain.
[0023] (3) in the present invention, papain concentration me...
Embodiment 2
[0025] (1) Add 2g of polyethylene glycol 400 to a 10ml centrifuge tube, and then add 4g of pre-configured NaH with a mass concentration of 60wt%. 2 PO4 -2H 2 O solution, followed by the addition of 1g of [C with a mass concentration of 40wt% 4 mim]Cl solution, followed by the addition of 1ml of pre-prepared papain solution with a mass concentration of 10mg / ml, and finally added to 10g with distilled water. In order to test the accuracy of the data, each sample was equipped with three parallel experiments.
[0026] (2) After the preparation of the two-phase aqueous system is completed, place it on a vortex shaker for 5 minutes to ensure uniform mixing of the entire system, and then put it in a constant temperature water bath at 25°C and let it stand for 24 hours. After the phase separation was completed, the upper and lower phase solutions were taken respectively to measure the concentration of papain.
[0027] (3) in the present invention, papain concentration measures ultr...
Embodiment 3
[0029] (1) Add 2g of polyethylene glycol 400 to a 10ml centrifuge tube, and then add 4g of pre-configured NaH with a mass concentration of 60wt%. 2 PO 4 -2H 2 O solution, followed by the addition of 1g of [C with a mass concentration of 40wt% 6 mim]Cl solution, followed by the addition of 1ml of pre-prepared papain solution with a mass concentration of 10mg / ml, and finally added to 10g with distilled water. In order to test the accuracy of the data, each sample was equipped with three parallel experiments.
[0030] (2) After the preparation of the two-phase aqueous system is completed, place it on a vortex shaker for 5 minutes to ensure uniform mixing of the entire system, and then put it in a constant temperature water bath at 25°C and let it stand for 24 hours. After the phase separation was completed, the upper and lower phase solutions were taken respectively to measure the concentration of papain.
[0031] (3) in the present invention, papain concentration measures ul...
PUM
Property | Measurement | Unit |
---|---|---|
extraction efficiency | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com