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Application of mogrol to anti-hepatic fibrosis drugs

A mogrosolic alcohol and liver fibrosis technology, applied in the field of natural medicine, can solve problems such as no application report of mogrosolic alcohol, and achieve the effects of clear activity, clear structure and wide range of extracts.

Inactive Publication Date: 2017-12-19
CHENGDU BIOPURIFY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the application of mogrosanol in anti-hepatic fibrosis drugs

Method used

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  • Application of mogrol to anti-hepatic fibrosis drugs
  • Application of mogrol to anti-hepatic fibrosis drugs
  • Application of mogrol to anti-hepatic fibrosis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Mogrosanol improves CCl 4 Induced liver fibrosis in mice

[0027] MG and Mogrol were used for the following in vivo pharmacodynamic studies. CCl 4 Inducing a mouse model of liver fibrosis is a classic approach and is similar to human liver fibrosis.

[0028] 1. Materials and Instruments

[0029] ICR mice, female, weighing 18-22 g, were provided by Nanjing Qinglongshan Animal Breeding Farm. Wahaha purified water; α-SMA monoclonal antibody (abcam); goat anti-rabbit IgG (bioworld).

[0030] 2. Experimental method

[0031]ICR female mice were divided into blank group, model group, MG group (250 mg / kg), and Mogrol group (10 mg / kg), and the drugs were administered by intragastric administration, 20 mice in each group. Mice were injected intraperitoneally with 40% CCl4 (olive oil suspension) 2ml / kg, twice a week to establish a mouse model of liver fibrosis; the blank group was injected intraperitoneally with olive oil 2ml / kg, twice a week. Drugs were administe...

Embodiment 2

[0042] Example 2 Effect of Mogrosanol on the Expression of α-SMA Protein in HSC-T6 Induced by TGF-β1

[0043] HSC-T6 is an effector cell of liver fibrosis. Transforming growth factor-β (TGF-β) is a key factor in promoting liver fibrosis, which can activate HSC-T6, and the activated HSC-T6 can release cytokines through autocrine or paracrine, and continuously accumulate a large amount of ECM, eventually causing hepatic fibrosis. fibrosis. In this experiment, the degree of liver fibrosis was judged by detecting the content of α-SMA protein.

[0044] HSC-T6 was purchased from Nanjing Kaiji Biotechnology Development Co., Ltd. HSC-T6 adopts DMEM medium containing 10% domestic calf serum at 37°C, 5% CO 2 cultured in an incubator. The medium was changed every 2 days, and the cells were digested with 0.25% trypsin when the density was 90%, and the cells were collected and passaged.

[0045] Take logarithmic growth cells, digest with trypsin, and adjust the cell concentration to 2...

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Abstract

The invention specifically relates to application of mogrol to preparation of drugs or / and health products used for preventing or / and treating hepatic fibrosis, belonging to the field of crude drugs. Experimental results prove that mogrol can substantially inhibit deposition of hepatocyte collagen, suppress hyperplasia of hepatic fibroblasts, reduce secretion of alpha-SMA in hepatic tissue and substantially improve hepatic fibrosis. Mogrol is definite in structure, good in stability, easily controllable in quality and cost, derived from the extract of natural plants, clear in activity, low in toxicity and side effect, provides a novel option and means for treatment or / and prevention of hepatic fibrosis, and has good practical value.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to a new medical application of mogrosvenol for resisting liver fibrosis. Background technique [0002] Hepatic fibrosis (hepatic fibrosis, HF) refers to the excessive deposition of diffuse extracellular matrix (especially collagen) in the liver. Early reversible stage. If left untreated, may progress to decompensated cirrhosis with various complications of end-stage liver disease. Continuous development of liver fibrosis can lead to cirrhosis and even liver cancer. Inflammation and necrosis of hepatocytes are the initiating factors of liver fibrosis and the driving factors of its development. Because its mechanism has not been clarified, therefore, it is subject to certain restrictions in terms of therapeutic drugs, and traditional Chinese medicine has the advantages of overall regulation and clear anti-oxidation, enzyme-reducing liver protection, low toxicity, and lo...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61P1/16A61P35/00
CPCA61K31/575A61K36/42A61P1/16A61P35/00
Inventor 谢海峰张朝凤胡云岭谢期林罗明锋
Owner CHENGDU BIOPURIFY LTD
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