Passage preservation method for coal geology microbial florae

Abstract

The invention belongs to the technical field of microbe preservation, and provides a passage preservation method for coal geology microbial florae in order solve a problem that the great demand for microbe sources in experiments cannot be met due to the severe reduction of the methane gas production activity of activated coal geology microbial florae preserved through existing strain preservation methods. The method disclosed in the invention comprises the following steps: 1, sampling; 2, inoculating; 3, carrying out 30 DEG C constant-temperature culture: drawing a periodic gas production curve map according to the pressure change, wherein one gas production period comprises the following three stages: the first stage starts from culture and lasts for 8-12 days, the second stage starts at day 10-12 and lasts until day 38-42, and the third stage is after the day 42; 4, allowing the methane content to be stable in the third stage of the culture, discharging half of the obtained fermentation solution, feeding the same volume of a sterilized medium, carrying out continuous culture until the third stage is reached, and feeding the medium again; and 5, repeating the strain culture. The method can be used to preserve the florae having a good gas production effect, especially strains in coal-bed gas well faced with low water, allows the florae to be directly used as a microbe source in other experiments without activation, and avoids frequent going up to hills and sampling.

Description

technical field [0001] The invention belongs to the technical field of microbial preservation, and in particular relates to a method for successive preservation of coal geological microbial flora, especially capable of long-term preservation at normal temperature. Background technique [0002] Bacteria are one of the key factors in carrying out coal biogasification experiments, because the bacteria source used in coal biogasification is a mixed flora, including at least four types of bacteria: hydrolyzing bacteria, fermenting bacteria, hydrogen-producing acetogenic bacteria and methanogenic bacteria Microorganisms, the physiological and biochemical characteristics of various microorganisms are different. The result of the general cryopreservation method is that the activated bacteria source is reused for coal biogasification, and the gas produced has only hydrogen and no methane, which is not suitable for coal geological microorganisms. Preservation of flora. [0003] Coal ...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem that the coal geological microbial flora is preserved by the existing strain preservation method, the activity of the activated coal geological microbial flora to produce methane gas is seriously reduced, and it cannot meet the large demand for bacterial sources in the experiment, and provides A method for subculture preservation of coal geological microbial flora. This method can not only realize the function of flora preservation, but also meet the large demand for bacterial sources in experiments. The strains preserved by this method do not need to be activated and can be directly used for inoculation and other experiments.

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