Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Technology for separating and purifying total polyphenols in Acanthopanax trifoliatus leaves

A technology for separation and purification of total polyphenols, which is applied in the field of separation and purification of total polyphenols, and can solve problems that cannot be truly applied

Inactive Publication Date: 2017-12-15
ZHEJIANG SCI-TECH UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these experiments have verified the role of macroporous resins in separation and purification, the contents are all below 15%, which cannot be really applied to actual operations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Technology for separating and purifying total polyphenols in Acanthopanax trifoliatus leaves
  • Technology for separating and purifying total polyphenols in Acanthopanax trifoliatus leaves
  • Technology for separating and purifying total polyphenols in Acanthopanax trifoliatus leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] 1. Materials and Reagents

[0041] Bougainvillea leaves: collected in Fenghuang Mountain (dried) in Guangdong Province, the thin stems and leaves were taken separately, and crushed for later use. Chlorogenic acid standard substance: self-made in the laboratory, the purity is over 98% verified by HPLC-DAD. Absolute ethanol and petroleum ether were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. Folin-Ciocalteu reagent was of analytical grade and was purchased from Shanghai Ruiyong Biotechnology Co., Ltd. Anhydrous sodium carbonate, hydrochloric acid, sodium hydroxide, etc. were purchased from Gaojing Chemical Co., Ltd. for analytical grade. ABTS, DPPH, VC, and VE are analytically pure with a purity of 98%, purchased from Shanghai Macklin Biochemical Technology Co., Ltd. Potassium persulfate was purchased from Tianjin Yongda Chemical Reagent Co., Ltd. for analytical grade. Experimental water: distilled water, self-made in the laborator...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a technology for separating and purifying total polyphenols in Acanthopanax trifoliatus leaves, and belongs to the technical field of medicinal preparations containing components coming from plants. The technology comprises the following steps: loading Acanthopanax trifoliatus leaf total polyphenol crude extract used as a loading solution to a chromatography column filled with purifying macro-porous resin, and controlling the concentration of the sample solution to be 0.5-10 mg / mL, the pH value of the sample solution to be 2-6 and the flow velocity to be 1-3 mL / min; and desorbing the sample solution with an eluent which is an ethanol-water solution with the volume concentration of 10-90%, the pH value of 2-7 and the speed of 1-3 mL / min after the sample loading is finished, and carrying out concentration and vacuum freeze-drying on a desorption solution obtained after the desorption to obtain a polyphenol sample. The technology is applied to the separation and purification of polyphenols in stems and leaves of Acanthopanax trifoliatus and like plants, and has the advantages of high purification efficiency, and high activity in the polyphenols.

Description

technical field [0001] The invention relates to a process for separating and purifying total polyphenols in leaves of Bougainvillea, and belongs to the technical field of pharmaceutical preparations containing plants. Background technique [0002] Caffeoylquinic acid is a compound widely present in plants and has pharmacological activities such as anti-oxidation, anti-inflammation, and antibacterial. It is a compound formed by condensation reaction of one molecule of quinic acid and multiple caffeic acids. Zhang et al. determined the content of flavonoids and caffeic acid quinine and other phenolic substances on 24 species of Aralis genus, and confirmed that the leaves of Bougainvillea from different origins are rich in caffeoylquinic acid polyphenols. Kiem et al. reported for the first time that the phenylpropanoid glycosides isolated from the stem bark of Bougainvillea were 1-β-D-glucopyranosyl-2,6-dimethoxy-4-propynyl-ene Alcohol (1) and 1-[β-D-glucopyranosyl-(1→6)-β-D-g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/254A61P39/06A61K127/00
CPCA61K36/254A61K2236/333A61K2236/51A61K2236/55
Inventor 张晓丹杨东风梁宗锁李小龙冯彩霞张岩
Owner ZHEJIANG SCI-TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com