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Lysinibacillussphaericus and application thereof in preventing and controlling meloidogyne incognita

A technology of Bacillus lysine and M. incognita, applied in the directions of application, pest control, nematicides, etc., can solve the problems of lack of bio-control preparations for root-knot nematodes, etc. High application value, the effect of overcoming environmental impact

Active Publication Date: 2017-12-12
宁夏鲲雨肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, there is still a lack of stable and efficient root-knot nematode biocontrol agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: In Vitro Screening of M. incognita Biocontrol Bacteria--Preliminary Screening of Hydrolyzed Gelatin Activity

[0026] The isolated 59 plant endophytic bacteria were streaked and activated on the NA plate, and cultured at 28°C for 48h. Pick a single colony and inoculate in 5mL NB medium (beef extract 0.3% (w / v), peptone 1% (w / v), NaCl 0.5% (w / v), pH 7.0-7.2, sterilize at 121°C for 20min ), 180rpm, cultured at 28°C for 12h to make seed solution. Pipette 5mL seed solution and inoculate in 50mL NB medium, culture at 180rpm, 28°C for 24h. The bacterial culture solution was centrifuged at 5000rpm for 20min to remove the supernatant, and the bacterial cells were washed twice with sterile distilled water and resuspended. Dilute the concentration of the bacterial suspension to 1.34×10 by dilution coating method 8 CFU / mL. Bacterial suspensions were serially diluted and spread on gelatin medium (gelatin 0.5% (w / v), K 2 HPO 4 0.05% (w / v), MgSO 4 ·7H 2 O 0.02% (w...

Embodiment 2

[0027] Example 2: In Vitro Screening of M. incognita Biocontrol Bacteria--Screening of Nematicidal Activity

[0028]The 19 strains obtained in Example 1 were cultured on the bacterial NB medium with hydrolyzed gelatin for 60 h, collected the supernatant after centrifugation and filtered twice with a 0.22 μm microporous membrane to sterilize again, and diluted the supernatant 5 times with sterile distilled water . Pipette 200 μL of supernatant into a 24-well plate, add 10 μL of nematode suspension (containing about 50 second-instar larvae of Meloidogyne incognita) to each well, and incubate at 25° C. for 12 hours. Add 10 μL of 1% NaCl to each well to stimulate the nematodes, observe the survival of the nematodes under a microscope (40X), the live nematodes are soft and curved, and the dead nematodes are stiff after stimulation. The number of live and dead nematodes was counted and the corrected lethality of the fermentation supernatant to the second instar larvae was calculate...

Embodiment 3

[0029] Embodiment 3: Pot control effect determination of root-knot nematode biocontrol bacteria in incognita

[0030] Tomato seeds with plump and consistent seeds were selected and planted in seedling pots filled with sterilized organic matter, and cultivated in a greenhouse at 28°C / 20°C, 14h light / 10h dark (day / night) and humidity 70%. After growing 2 true leaves, transplant them into flowerpots containing 1000g of nutrient soil. The nutrient soil is mixed with sterilized healthy loam and organic matter 1:1 (v:v). The upper diameter of the flowerpot is 15cm, and the height is 14cm. Three tomato seedlings were transplanted in pots. After slowing down the seedlings for one week, inoculate 10mL of the bacterial suspension to each wounded root, that is, use a knife to cut a 3-5cm deep groove on the side of the seedling at a distance of 1cm from the root to cut the root, and pour the bacterial suspension to the cut root side. In the same way, 10 mL of sterile distilled water was ...

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PUM

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Abstract

The invention discloses a lysinibacillussphaericus separated from tomato roots and an application thereof as a meloidogyne incognita biocontrol bacterium. The bacterial strain is identified as Lysinibacillussphaericus; the name of the bacterial strain is RQ11; the bacterial strain is collected at China General Microbiological Culture Collection Center; the collection number is CGMCC No.14201; the collection date is May 27th, 2017; and the bacterial strain has an excellent lethal property for the meloidogyne incognita and also can obviously promote the growth of the tomato. The lysinibacillussphaericus RQ11 bacterial strain acquired according to the invention is an endophytic bacterium colonized in the tomato roots, is difficult to be influenced by the outside environment and overcomes the environmental influence of soil change on the biocontrol bacterium. The RQ11 has higher application value at the aspect of preventing and controlling the meloidogyne incognita, especially is the endophytic bacterium of tomato and is capable of continuously acting on the meloidogyne incognita, so that the defect of low effect or instability caused by a general biocontrol bacterium incapable of colonizing in the root can be overcome.

Description

technical field [0001] The invention belongs to the technical field of microbial biology, and in particular relates to a lysine bacillus sphaericus and its application in preventing and treating root-knot nematode incognita. Background technique [0002] Plant parasitic nematodes are one of the important pathogens of plant infestation diseases, which occur widely in the world and cause serious damage. Root-knot nematode is a kind of plant-parasitic nematode that seriously endangers agricultural production. It mainly parasitizes on more than 2,000 hosts such as vegetables, food crops, cash crops, fruit trees, ornamental plants and weeds. Tropical, subtropical and temperate plants are particularly damaged. , after the disease occurs, the yield is generally reduced by about 10%, and in severe cases, it is as high as more than 75%. In particular, root-knot nematode incognita has a wide incidence area and can infect almost all cultivated plants. Root-knot nematodes mainly damag...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12R2001/07C12N1/205
Inventor 闫淑珍陈双林王玉芳王聪
Owner 宁夏鲲雨肥业有限公司
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