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A kind of SNP molecular marker related to multiple lambs in sheep and its application

A molecular marker and sheep technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of difficult high-throughput automatic measurement, low throughput, and numerous procedures, so as to improve reproduction Force, high accuracy, technically sensitive effects

Active Publication Date: 2021-02-02
内蒙古河套农牧业技术研究院 +1
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AI Technical Summary

Problems solved by technology

Most of the traditional genotype detection methods use PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) detection methods. Difficult to achieve high-throughput automated assays

Method used

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  • A kind of SNP molecular marker related to multiple lambs in sheep and its application
  • A kind of SNP molecular marker related to multiple lambs in sheep and its application
  • A kind of SNP molecular marker related to multiple lambs in sheep and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 A kind of utilizing Sequenom Method for detecting HIRA genotype of sheep and predicting average litter size of multiparous ewes

[0041] 1. Experimental materials

[0042] 375 small-tailed Han sheep were selected as the test objects.

[0043] 2. Reagents and instruments

[0044] Reagent: Complete Genotyping Reagent Kit for Compact 384;

[0045] Gene Amplification: ABI 9700 384Dual;

[0046] Mass spectrometry spotting: MassARRAY NanodispenserRS1000;

[0047] Mass spectrometry: MassARRAY Compact System;

[0048] All reagents and instruments were purchased from Beijing Genenode Biotech Co., Ltd.

[0049] 3. Genomic DNA extraction

[0050] 1ml of blood was collected from the jugular vein of the sheep and treated with EDTA anticoagulant. First, the red blood cell lysate is lysed to remove DNA-free red blood cells, the nucleus lysate is used to lyse the cells to release genomic DNA, and then the protein precipitation solution is selectively precipitate...

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Abstract

The present invention relates to molecular marker detection technology, and specifically discloses a SNP molecular marker related to multiple lambs in sheep and its application. By analyzing the 71874104bp site on the No. 17 chromosome of sheep (XM_015101463.1, based on the version number of the sheep genome sequence information Oar_v4.0, December 2015) was tested for single nucleotide type, and the sheep HIRA gene was determined to be homozygous or heterozygous according to the test results, and the average litter size of multiparous ewes was predicted. Using this method, automatic detection of SNP sites of histone regulation (HIRA) genes can be realized. By selecting the HIRA genotype of sheep, AG heterozygous individuals and GG homozygous individuals with high litter size traits can be selected, thereby improving the fecundity of sheep, which has potential application value for large-scale molecular breeding of sheep.

Description

technical field [0001] The invention relates to molecular marker detection technology, in particular to a SNP molecular marker related to multiple lambs in sheep and its application. Background technique [0002] The litter size trait is one of the most important economic traits of sheep. Because it is controlled by micro-effect polygenes and has low heritability, it is difficult to improve rapidly by conventional breeding methods. Molecular technology can effectively and rapidly improve its genetic progress, so the identification Major genes or molecular genetic markers related to litter size are the key to modern molecular breeding. [0003] The HIRA gene is located on chromosome 17 of sheep, contains 27 exons, the total length of the coding region is 3276bp, and the encoded protein contains 1091 amino acids. source. In addition, tissue-specific expression analysis indicated that this gene may play a role in maintaining ovarian function. Nashun et al. found that HIRA pl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 储明星刘秋月周梅狄冉王翔宇胡文萍
Owner 内蒙古河套农牧业技术研究院
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