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Method for preparing erythritol with bean dregs as main raw material

A main raw material, a technology for erythritol, applied in the field of preparing erythritol, can solve the problems of difficulty in realizing full hydrolysis and utilization of bean dregs, high protein content, high carbon-nitrogen ratio, and inability to hydrolyze bean dregs cellulose, etc. Synthetic, Nutritious, Inexpensive Effects

Active Publication Date: 2017-12-08
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, bean dregs are used as Y. lipolytica There are still two disadvantages in fermented raw materials: one. Y. lipolytica It does not have the ability to secrete cellulase, and cannot hydrolyze cellulose, the main component of bean dregs, so it is difficult to fully hydrolyze and utilize bean dregs, and it is easy to cause secondary pollution; 2. The high protein content in bean dregs leads to an excessively high carbon-nitrogen ratio. Not conducive to product synthesis in microbial fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. After the bean dregs are dried and dehydrated, the moisture content is controlled at 60% by adding distilled water. According to the inoculum size is 1×10 8 cells / 100g dry bean dregs A. elegans The spores are mixed well, fermented at 30°C for 5 days, sterilized at 115°C for 30 minutes after pre-fermentation, and dried at 60°C.

[0026] 2. The amount of pre-fermented dried okara is 30.0 g / L, and potassium chloride is used as the osmotic pressure regulator, and the added concentration is 20 g / L. The inoculum size is 1×10 6 cells / liter medium; culture temperature 26°C; stirring speed 140 rpm; pH was not adjusted during the fermentation process. The yield of erythritol was determined to be 0.41 g / g pre-fermented okara.

Embodiment 2

[0028] 1. After the bean dregs are dried and dehydrated, the moisture content is controlled at 30% by adding distilled water. According to the inoculum size is 1×10 5 cells / 100g dry bean dregs A. elegans The spores are mixed well, fermented at 26°C for 3 days, sterilized at 115°C for 30 minutes after pre-fermentation, and dried at 60°C.

[0029] 2. The amount of pre-fermented dried bean dregs was 30.0 g / L, and sodium chloride was used as an osmotic pressure regulator at a concentration of 40 g / L; the inoculation amount was 1×10 8 cells / liter of medium Y. lipolytica Cell concentration; culture temperature 30°C; stirring speed 200 rpm; pH was always controlled at 3.0 during the fermentation process. The yield of erythritol was determined to be 0.30 g / g pre-fermented okara.

Embodiment 3

[0031] 1. After the bean dregs are dried and dehydrated, the moisture content is controlled at 30% by adding distilled water. According to the inoculum size is 1×10 5 Cells per 100 grams of dried bean dregs A. elegans The spores are mixed well, fermented at 26°C for 3 days, sterilized at 115°C for 30 minutes after pre-fermentation, and dried at 60°C.

[0032] 2. The amount of pre-fermented dried bean dregs was 30.0 g / L, and sodium chloride was used as an osmotic pressure regulator at a concentration of 40 g / L; the inoculation amount was 1×108 cells per liter of medium Y. lipolytica Cell concentration; culture temperature 30°C; stirring speed 200 rpm; pH was adjusted to 3.0 48 hours before fermentation, and adjusted to pH 5.0 after 48 hours. The yield of erythritol was determined to be 0.39 g / g pre-fermented okara.

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Abstract

The invention provides a method for preparing erythritol with bean dregs as a main raw material. The method comprises the following steps: firstly, pre-fermenting bean dregs by utilizing actinomucor elegans (A. elegans), and by controlling the pre-fermenting condition, sufficiently hydrolyzing macromolecular substances in the bean dregs by utilizing an enzyme system generated by the A.elegans, and reducing utilization of the A.elegans for nutritional substances as much as possible so as to furthest ensure the solubility and utilization of macromolecular substances in the bean dregs; and performing erythritol liquid-state fermentation by using erythritol production strain Y.lipolytica as a fermenting strain and the pre-fermented bean dregs as the unique matrix.

Description

technical field [0001] The invention relates to the technical field of biochemical industry, in particular to a method for preparing erythritol from bean dregs as a main raw material. Background technique [0002] Erythritol, also known as 1,2,3,4-butitol, is a low-calorie sweetener with a caloric value of only 0.3 kcal / g, far lower than other sweeteners, and its sweetness can reach 70~80% of sucrose, so it is the most ideal dietary supplement for obese patients. Erythritol cannot be metabolized by the human body. 90% of the ingested erythritol will not be absorbed but will be excreted directly with the urine. Therefore, the glycemic index is extremely low, and it is suitable for people with diabetes and cardiovascular diseases. In addition, erythritol cannot be utilized by caries-causing bacteria and can replace xylitol in oral care. And animal toxicology and clinical studies have proved that erythritol is a safe sweetener. Based on the above properties, erythritol has b...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/785C12R1/645
CPCC12P7/18
Inventor 刘晓燕吕金顺徐继明许家兴夏军贺爱永张瞳
Owner HUAIYIN TEACHERS COLLEGE
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