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Insect-resistant soil regulator taking traditional Chinese medicine wastes as matrix and production method of soil regulator

A technology of soil conditioner and production method, which is applied in the field of soil conditioner, can solve the problems of short survival time of mycelia, low survival rate, and a large amount of waste, and achieve the effects of easy control and amplification, inhibition of insect reproduction, and elimination of odor Effect

Inactive Publication Date: 2017-12-08
MICROBIOLOGY INST OF SHAANXI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solid-phase fermentation of Metarhizium anisopliae also has problems such as the production of a large amount of waste after fermentation, the short survival time of mycelium and the low survival rate.

Method used

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  • Insect-resistant soil regulator taking traditional Chinese medicine wastes as matrix and production method of soil regulator
  • Insect-resistant soil regulator taking traditional Chinese medicine wastes as matrix and production method of soil regulator
  • Insect-resistant soil regulator taking traditional Chinese medicine wastes as matrix and production method of soil regulator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Step 1: inoculating Metarhizium anisopliae into the seed medium to obtain a fermented seed liquid;

[0034] The formula of the seed medium is: by weight of the medium, glucose 2%, yeast powder 0.2%, peptone 0.2%, 1000ml contains 200g potato liquid boiled for 30min, pH 6.0.

[0035] Step 2: inoculate the fermented seed solution prepared in step 1 on a solid-phase medium with an inoculum size of 15%, and place it in a shallow plate solid-state fermentation device at 25°C for static fermentation;

[0036] The formula of the solid phase medium is: according to the weight of the medium, 50% of traditional Chinese medicine waste, 5% of sucrose, 1% of corn steep liquor powder, 30% of soybean meal, Ca(OH) 2 1%, 30% wood vinegar diluted with water at a ratio of 1:300.

Embodiment 2

[0038] Step 1: inoculating Metarhizium anisopliae into the seed medium to obtain a fermented seed liquid;

[0039] The formula of the seed medium is: by weight of the medium, glucose 2%, yeast powder 0.2%, peptone 0.2%, 1000ml contains 200g potato liquid boiled for 30min, pH 6.1.

[0040] Step 2: inoculate the fermented seed liquid prepared in step 1 on a solid-phase medium with an inoculum size of 12%, and place it in a shallow-pan solid-state fermentation device for static fermentation at 27°C;

[0041] The formula of the solid-phase medium is: by weight of the medium, Chinese medicine waste 50%, sucrose 3%, corn steep liquor 2%, soybean meal 20%, Ca(OH) 2 2%, 30% wood vinegar diluted with water at a ratio of 1:180.

Embodiment 3

[0043] Step 1: inoculating Metarhizium anisopliae into the seed medium to obtain a fermented seed liquid;

[0044] The formula of the seed medium is: by weight of the medium, glucose 2%, yeast powder 0.2%, peptone 0.2%, 1000ml contains 200g potato liquid boiled for 30min, pH 6.3.

[0045] Step 2: inoculate the fermented seed solution prepared in step 1 on a solid-phase medium with an inoculum size of 10%, and place it in a shallow plate solid-state fermentation device at 30°C for static fermentation;

[0046] The formula of the solid-phase medium is: by weight of the medium, Chinese medicine waste 50%, sucrose 2%, corn steep liquor 3%, soybean meal 10%, Ca(OH) 2 3%, wood vinegar 30% diluted with water at a ratio of 1:60.

[0047]This product was used for demonstration in Nangou Village, Ansai District, Yan'an. The addition amount was 1t per mu, and it was used as a base fertilizer to plant corn. Compared with the control group (CK), the incidence of lepidopteran pests such a...

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Abstract

The invention relates to an insect-resistant soil regulator taking traditional Chinese medicine wastes as a matrix and a production method of the soil regulator. The production method comprises the following steps: inoculating green muscardine fungus to a seed medium, and preparing a fermentation seed solution, wherein the seed medium comprises glucose, yeast powder, peptone and a potato liquid; and inoculating the fermentation seed solution to a solid-phase medium, wherein the inoculation amount is 10-15%, and standing and fermenting in a shallow tray solid fermentation apparatus at the temperature of 25-30 DEG C, wherein the solid-phase medium comprises the traditional Chinese medicine wastes, saccharose, maize syrup powder, bean pulp, Ca(OH)2 and wood vinegar. The fermentation method in the invention is short in fermentation cycle, mild in conditions and easy to control and amplify, the provided solid-phase fermentation matrix is high in fermentation rate, and the fermented soil regulator has an insect-resistant effect; and meanwhile, the functional substance wood vinegar can provide the characteristics of promoting growth, resisting diseases and the like.

Description

technical field [0001] The invention relates to a microbial fermentation soil conditioner, in particular to an insect-resistant soil conditioner based on traditional Chinese medicine waste and a production method thereof. Background technique [0002] my country is a country that consumes a large amount of traditional Chinese medicine resources, and a large amount of traditional Chinese medicine waste is mainly generated from the production process of various traditional Chinese medicines. Among them, the residues left in the production process of Chinese patent medicines are the most, accounting for about 70% of the total amount of traditional Chinese medicine residues. At present, there are about 1,636 Chinese medicine companies in my country, accounting for 32% of the national pharmaceutical industry enterprises, including 1,283 Chinese patent medicine companies, and about half of the top 30 pharmaceutical companies rely on the Chinese medicine industry. These large numbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C05G3/00C05G3/02C05G3/04C12R1/645C05G3/60C05G3/80
CPCC05D3/02C05G3/00C12P1/02C05G3/60C05G3/80C12N1/145C12R2001/645C05F5/002C05F11/00C05F11/08
Inventor 贾凤安陈怡平常帆吕睿上官亦卿
Owner MICROBIOLOGY INST OF SHAANXI
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