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A sio for detecting malachite green 2 Preparation method of @rox nanoparticle fluorescent probe array

A nanoparticle and fluorescent probe technology, which is applied in the field of material science, can solve the problems of airgel size control, recycling problems, and no recognition groups, etc., to achieve difficult molding, reduce production costs, and improve production safety sexual effect

Active Publication Date: 2019-08-16
HEFEI UNIV +1
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, during the preparation process SiO 2 The size of airgel is not easy to control, and there are recycling problems in practical applications
[0010] Although the above method has many advantages, there is no report in the literature that the emission band is red fluorescent probe and its surface is post-functionalized, so it does not carry a specific recognition group.
At the same time, there is no literature report on the detection of trace amounts of malachite green by modifying aminopropyl groups on the surface of fluorescent probes

Method used

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  • A sio for detecting malachite green <sub>2</sub> Preparation method of @rox nanoparticle fluorescent probe array
  • A sio for detecting malachite green <sub>2</sub> Preparation method of @rox nanoparticle fluorescent probe array
  • A sio for detecting malachite green <sub>2</sub> Preparation method of @rox nanoparticle fluorescent probe array

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preparation example Construction

[0046] A kind of SiO that detects malachite green 2 The preparation method of @ROX nanoparticle fluorescent probe array is characterized in that: the SiO 2 The surface of the fluorescent particles of @ROX has aminopropyl groups, and the aminopropyl groups on the surface can further interact with malachite green target molecules, making the electron-rich aminopropyl groups on the surface act as electron donors and electron-deficient malachite green acceptors. A blue-green complex is formed between them through charge transfer, and this blue-green complex can absorb SiO through fluorescence resonance energy transfer 2 The red fluorescence of the @ROX nanoparticle fluorescent probe array uses the change of fluorescence intensity to realize the detection of malachite green. The SiO 2 The preparation process of @ROX fluorescent probe includes the following three steps:

[0047] The first step is the preparation of the ROX-APTS precursor: First, accurately measure 10 mL to 12 mL o...

specific Embodiment

[0052] According to the covalent coupling reaction, the ROX-APTS conjugate was prepared as a precursor, and the surface of SiO rich in aminopropyl groups could be synthesized by sol-gel reaction 2 @ROX Nanoparticle Fluorescent Probes Prepared on SiO by Plasma Etching 2 @ROX nanoparticle fluorescent probe array.

[0053] The first step is the preparation of the ROX-APTS precursor: First, accurately measure 11 mL of absolute ethanol, 2 mL of 3-aminopropyl triethoxysilane (3-aminopropyl triethoxysilane, APTS), and 200 μL of 5-carboxy -X-rhodamine succinimidyl ester (5-Carboxy-X-rhodamine N-succinimidyl ester, ROX) was placed in a 50 mL single-neck ground flask, then 2 mL of deionized water was added, and the above single-neck ground The flask was stirred at 600 rpm to 700 rpm at room temperature for 11 h in the dark, and finally a red solution of ROX-APTS precursor containing excess unreacted APTS was obtained;

[0054] The second step is the surface modification of ROX dyes an...

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Abstract

The invention discloses a method for preparing a SiO2@ROX nanoparticle fluorescent probe array for detecting malachite green. The method comprises the following steps: coupling APTS (3-Amimopropyl Triethoxysilane) and an organic dye ROX (5-Carboxy-X-Rhodamine N-Succinimidyl Ester), and dehydrating with TEOS (Tetraethylorthosilicate) under an alkali condition to synthesize SiO2@ROX nanoparticles of surface functionalized modified aminopropyl; and preparing the SiO2@ROX nanoparticle fluorescent probe array on an etched silicon wafer. The method comprises the following three steps: firstly, preparing an ROX-APTS precursor in an ethanol system, performing functionalized modification on aminopropyl and an ROX dye onto the surfaces of SiO2 nanoparticles by using a sol-gel method, and finally dropping a SiO2@ROX nanoparticle fluorescent probe onto a silicon wafer etched with micro-pores, and removing excessive particles on the surface, thereby obtaining the SiO2@ROX nanoparticle fluorescent probe array which is large in specific surface area, rich in recognition site and good in sensitivity for selective recognition and trace detection on a target analyte, namely malachite green.

Description

technical field [0001] The present invention relates to the field of material science, in particular to SiO with trace malachite green detection 2 Preparation method of @ROX nanoparticle fluorescent probe array. . Background technique [0002] Malachite green (MG) metabolizes rapidly in organisms to produce fat-soluble leuco malachite green, which has hazards such as refractory, high toxicity, high residue, high carcinogenicity, high teratogenicity and mutagenicity, and has been approved by many countries. Listed as a banned drug for aquaculture. Therefore, the detection of ultra-trace triphenylmethane compounds and the study of related fluorescent probe arrays have attracted extensive attention and fruitful explorations from social research institutions. The laboratory detection of triphenylmethane substances such as malachite green has been widely used by enzyme-linked immunoassay, liquid chromatography, high performance liquid chromatography, and thin-layer chromatogra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 高大明侯晶晶苏学军王中华席小倩漆天瑶周杨群陈红
Owner HEFEI UNIV
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