Fructus psoraleae medicinal material detection method based on quantitative analysis of multi-components by single marker

Abstract

The invention provides a fructus psoraleae medicinal material detection method based on quantitative analysis of multi-components by single marker. The fructus psoraleae medicinal material detection method comprises the following steps: firstly, establishing an HPLC (High Performance Liquid Chromatography) content determination method for 10 main medicinal components in fructus psoraleae; taking psoralen as an internal-standard reference object, calculating relative correction factors of the other 9 active components and considering system applicability and method repeatability of the relative correction factors; carrying out chromatographic peak positioning according to relative retention time; calculating the content of each component to be determined by combining using the relative correction factors and proving that results have no remarkable difference through mutual verification of the quantitative analysis of multi-components by single marker and an external-standard method. By adopting the method provided by the invention, the difficulties that a reference substance has low the defect of high cost and is not easy to obtain and the like are overcome; the content of index components is calculated through the relative correction factors and the chromatographic peak positioning and synchronous determination of various medicinal substances in the fructus psoraleae is realized; the cost can be saved and the operation can be simplified; the efficiency is improved, the detection sensitivity is high, the stability is good and a determination result is accurate and reliable; the fructus psoraleae medicinal material detection method provided by the invention has great significance of in controlling the quality of the fructus psoraleae and ensuring the clinical effect.

Description

Technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a method for detecting Chinese medicine psoralen medicinal materials, and in particular to a method for detecting psoralen medicinal materials based on a one-test and multiple-evaluation method. Background technique [0002] The components of traditional Chinese medicine are complex and changeable, and its pharmacological activity and clinical efficacy are often the result of the joint action of multiple components. Taking the content of a single component or a certain type of component as an evaluation index usually cannot fully and fully reflect the quality of Chinese medicine. To control the quality of traditional Chinese medicine by measuring the content of multiple components, there are many problems such as the scarcity, instability, and high cost of reference materials, which also causes great difficulties in testing. If the content of a certain component is d...

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Problems solved by technology

At present, in domestic and foreign researches, the content determination of psoralen and isopsoralen is directly carried out by external standard method, and the content determination of other components in psoralen such as flavonoids is also determined by the method of one test and multiple evaluation. There are no relevant reports on the one-test and multiple-evaluation method for benzofuranosides (psoralen, isopsoralen) in psoralen

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