Broiler growth character-associated molecular marker and application thereof

A technology of molecular markers and growth traits, applied in the field of poultry genetics and breeding, to achieve the effect of improving production performance

Active Publication Date: 2017-11-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no information about chickens in the prior art c-Myc Report of Genes as Molecular Markers for Chicken Growth Traits

Method used

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  • Broiler growth character-associated molecular marker and application thereof
  • Broiler growth character-associated molecular marker and application thereof
  • Broiler growth character-associated molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for selecting and breeding medium-speed type broilers using molecular markers related to broiler growth traits, comprising the steps of:

[0028] 1. Collection of chicken blood samples and DNA extraction:

[0029] After the determination of the pedigree and growth traits of the tested chickens is completed, about 1 mL of blood is collected from the subwing vein, and injected into a 1.5 mL centrifuge tube that has been autoclaved and filled with 150 μL of 2% sterile EDTA (Ethylenediaminetetraacetic acid, EDTA) anticoagulant , shake gently, record the wing number, and store at -20°C for later use.

[0030] Genomic DNA was extracted using phenol-chloroform extraction (Osper F et al., 1998):

[0031] (1) Take 30 μL of whole blood and place in a 1.5 mL centrifuge tube, add 470 μL of 1×SET buffer, 12.5 μL of 20% SDS and 6 μL of 10 mg / mL proteinase K, mix well and place in a 55°C water bath overnight;

[0032] (2) Add 500 μL of Tris-saturated phenol to the digested ...

Embodiment 2

[0049] The present invention is c-Myc The correlation between gene SNP loci and chicken growth traits was detected and analyzed.

[0050] Pass the test c-Myc Gene g.1834T>C loci in South China Agricultural University xinghua×recessive white chicken F2 resource family population genotype, and association analysis was carried out according to the recorded growth traits.

[0051] The GLM program in SAS 9.2 software (SAS Institute, 1996) was used for correlation analysis, and its mathematical model is as follows:

[0052] Y ijkl = μ + G i +D j + H k +e ijkl

[0053] Y ijkl is the observed value of growth traits, μ is the population mean of growth traits, G i is the genotype effect, D j is the familial effect, H k is the batch effect, e ijkl is a random error effect.

[0054] c-Myc The results of the correlation analysis between the gene g.1834T>C site and chicken growth traits are shown in Table 2. From the table, it can be concluded that the g.1834T>C site is ...

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Abstract

The invention discloses a broiler growth character-associated molecular marker and an application thereof. A nucleotide sequence of the molecular marker is as shown in SEQ ID NO:1, and a T / C mononucleotide mutation is arranged at the 1834th bp of a second intron in the SEQ ID NO:1. The molecular marker can be applied to breeding of a medium-speed broiler and the application methodcomprises the steps of extracting and amplifying DNA of a to-be-detected broiler; carrying out sequencing and sequence alignment and typing on an amplified product; and selectively reserving an individual which is judged as a heterozygous genotype during breeding. The body weight of the heterozygous genotype individual screened through the molecular marker disclosed by the invention at D70, D77 and D84 is significantly higher than that of a homozygote individual, and the shin length at 77D and the shank circumference at 70D are also significantly higher than those of the homozygote individual. The molecular marker is beneficial to breeding of a dominant heterozygote broiler, and the production performance of the broiler is improved.

Description

technical field [0001] The invention relates to the technical field of poultry genetics and breeding, in particular to a molecular marker related to broiler growth traits and its application. Background technique [0002] my country's local breed broiler chicken resources are very rich, but compared with foreign fast and large broiler chickens, the production performance is lower. Growth is a very important economic trait in livestock and poultry production, so the growth traits of broiler chickens have always been the key research object of poultry breeding, and the analysis of growth traits is of great significance to the growth and breeding of broiler chickens. [0003] With the rapid development of modern molecular biology technology, the new technology can quickly and accurately analyze the genetic composition of livestock and poultry from the molecular level, so as to realize the direct selection of genotype, and use molecular markers to assist selection breeding. The...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 蒋明雅罗文张细权
Owner SOUTH CHINA AGRI UNIV
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