Tissue culture and rapid propagation method of flower and plant nursery stock

Abstract

The invention relates to the technical field of agricultural living things, in particular relates to a tissue culture and rapid propagation method of flower and plant nursery stock. The method takes stem apexes as explants, and comprises the following steps of carrying out sterilization on the explants, carrying out shoot inducing culture, carrying out proliferation culture, carrying out rooting and hardening-off culture, exercising seedlings, transplanting, and the like, wherein a disinfectant used for carrying out sterilization on the explants is prepared from the following raw materials: garlic, tea leaves, radix rubiae, folium artemisiae argyi, aloe, semen sophorae alopecuroidis, ampelopsis grossedentata, radix sanguisorbae, radix saposhnikoviae, starch paste, gum, castor oil, juniper oil, shikakai powder, tea seed cake powder, and the like. The tissue culture and rapid propagation method of flower and plant nursery stock is high in antibacterial efficiency and free from harm and pollution to tissues of the explants, and effectively increases the bud induction rate, rooting rate and survival rate of the flower and plant nursery stock so as to promote the propagation production of the flower and plant nursery stock.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of flower seedlings. Background technique [0002] Due to the limitation of many factors such as environment, season, soil and species itself, the breeding of flower seedlings is also limited. It is very difficult to realize its large-scale industrial production, and it is difficult to breed high-quality varieties. At present, most of the asexual reproduction methods such as conventional cuttings, layering or grafting are used in the market to breed flower seedlings, but the speed of these methods is slow, and the quality of the varieties bred is not good, which cannot meet the growing market demand. [0003] Plant tissue culture is based on the theory that plant cells are totipotent, using isolated plant organs (such as roots, stems, leaves, shoot tips, etc.), tissues (such as cambium, epidermis, cortex, pith cells, endo...

AI Technical Summary

Problems solved by technology

However, plant tissue culture technology is affected by factors such as medium nutrients, bacteria, and supports, which limits the production of flower seedlings. At this time, it is necessary to take corresponding measures to improve these problems, such as disinfecting explants to reduce bacteria. Contamination of the culture medium. At present, alcohol, calcium hypochlorite, and mercuric chloride are usually used for explant disinfection. Although bacteria on the surface of the explant can be eliminated, it will cause certain damage to the tissue of the explant, and mercuric chloride is toxic. It is difficult to clean and pollutes the environment; therefore, optimizing plant tissue culture technology is the key to the breeding of flower seedlings