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Secalonic acid I originated from penicillium oxalicum and application thereof in preparation of drugs for preventing human esophagus cancer

A technology of Penicillium oxalicum and esophageal cancer, applied in the field of medicine and biology, to achieve significant human esophageal cancer cell proliferation activity and the effect of inhibiting human esophageal cancer cell proliferation activity

Active Publication Date: 2017-11-17
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the indicated ryronic acid compounds have anti-human esophageal cancer activity, and there is no report on the anti-proliferation activity of this compound on human esophageal cancer cells, so there is no drug related to this in the market.

Method used

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  • Secalonic acid I originated from penicillium oxalicum and application thereof in preparation of drugs for preventing human esophagus cancer
  • Secalonic acid I originated from penicillium oxalicum and application thereof in preparation of drugs for preventing human esophagus cancer
  • Secalonic acid I originated from penicillium oxalicum and application thereof in preparation of drugs for preventing human esophagus cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium oxalicum ( Penicillium oxalicum ) IBPT-6 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013714) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 2 to 3 days.

[0022] Penicillium oxalicum ( Penicillium oxalicum ) Appropriate amount of IBPT-6, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.3, NaCl 15.0, constant volume] in a 1000mL Erlenmeyer flask, after static culture at 28°C for 30 days, the mycelia and fermentation broth w...

Embodiment 2

[0031] Example 2 Test of anti-tumor activity in vitro

[0032] 1 Experimental samples and experimental methods

[0033] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0034] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with DMEM medium containing 10% FBS at 37°C with 5% CO 2 Subculture in an incubator.

[0035] Cell Proliferation Inhibitory Activity Test Method

[0036] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 1×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2for 4 hours in an incubator. Add 2 microliters of sample solution...

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Abstract

The invention relates to secalonic acid I originated from penicillium oxalicum and an application thereof in the preparation of drugs for preventing human esophagus cancer. Secalonic acid can inhibit the proliferation of human esophagus cancer cells. The structure of secalonic acid is represented in the description. Penicillium oxalicum IBPT-6 is cultured through fermentation to obtain the fermentation product; and secalonic acid is separated from the fermentation product and then purified. The experiment results show that secalonic acid can well inhibit the activity of human esophagus cancer cell EC9706. Secalonic acid can be used to prepare drugs for inhibiting the proliferation of human esophagus cancer cells and antitumor drugs, and can also be applied to the research on human esophagus cancer.

Description

technical field [0001] The invention relates to the application of ryeconic acid I derived from Penicillium oxalicum and preparation of anti-human esophageal cancer medicine, and belongs to the field of medicine and biology. Background technique [0002] Secalonic acids belong to the secondary metabolites of Ergochrome and are xanthone dimers. Since Stoll et al. isolated Secalonic acid A (Secalonic acid A) from fungi in 1952, the series of compounds Secalonic acid (A, B, C, D, E, F, G) have been continuously identified. discovery and research. Secalonic acid compounds have various physiological activities. Taking Secalonic acid D (Secalonic acidD, SAD) as an example, 5 mg / ml of SAD is added to normal saline, within the range of 5-20 mg, that is It can treat early bladder cancer, within the range of 50-100 mg, it is effective for more serious bladder cancer and has no side effects. It has been found through research that some marine fungi can produce ryryronic acid compoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/80A61K31/352A61P35/00C12P17/06C12R1/80
CPCC07D311/80C12P17/06
Inventor 陈立王思远夏其文刘沁颖毕延雪伍久林张其清
Owner FUZHOU UNIV
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