Constructing method of practical model for evaluating hepatitis B hepatopathy NK cell functions

Abstract

The invention provides a constructing method of a practical model for evaluating hepatitis B hepatopathy NK cell functions. The method includes the steps of firstly, grouping hepatitis B hepatopathy patients into chronic hepatitis active stage patients and chronic hepatitis inactive stage patients, detecting the frequency distribution of peripheral blood NK cells and subsets of the peripheral blood NK cells through the flow cytometry, and carrying out comparison; secondly, detecting the level of the peripheral blood NK cells and cytokines secreted by the subsets of the peripheral blood NK cells through the flow cytometry, and carrying out comparison; thirdly, collecting clinical indexes of the patients, and conducting correlation analysis on the clinical indexes and NK cell immunity evaluation indexes; fourthly, using NK cell function activeness and NK cell hypofunction as the feedback variables, and screening out indexes correlated to NK cell function states of the patients through a multi-variable logistic regression model; fifthly, constructing the evaluating model; sixthly, constructing a nomogram.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a practical model for evaluating the function of NK cells of hepatitis B liver disease and a construction method thereof. technical background [0002] Natural killer (NK) cells are important immune cells in the body. Unlike T cells, their killing activity is not limited by MHC and does not depend on antibodies, so they are called natural killer cells; NK cells are used in fighting tumors and viral infections It plays an important role in immune regulation and is the first line of defense for the human body to defend against pathogen invasion and cell mutation in the body. According to the density of CD56 on the surface of NK cells, they can be divided into CD56 bright and CD56 dim Two subtypes. The functions of NK cells include: (a) Natural killer activity: through non-specific recognition of target cells and release of killing mediators, such as perforin (Perforin), NK...

AI Technical Summary

Problems solved by technology

However, in many patients with chronic liver disease and liver cancer, with the development of life omics, the technology related to the immune system has been continuously improved. Since the 1970s, the determination of immunoglobulin and complement can roughly and indirectly estimate the immune status of the body , has been gradually replaced by the detection of lymphocyte subsets and cytokines. Representative detection methods include: (1) biological methods based on DNA detection, such as PCR, northern blotting, in situ hybridization and other techniques. Single immune cytokines should be detected, and their regulatory pathways should be studied, but they should not be used to distinguish clinical complex cases; (2) biological activity detection methods, such as ELISA, ELISPOT, etc., these methods only detect single cells or factors secreted by single immune cells. Detection, rather than the detection of the average cytokine level of a certain group of immune cells, is also not conducive to the discrimination of clinical complex cases; (3) flow cytometry and liquid chip detection: this is currently more accurate detection technology, Multiple cytokines can be detected, and clinicians can estimate the patient's immune status based on different cytokine levels, but it does not involve the overall function evaluation of immune cells

[0006] It can be seen that all the above detection techniques do not involve the evaluation of the overall function of immune cells, and cannot detect multiple cytokines at the same time, so that clinicians can estimate the immune status of patients according to different cytokine levels, and have a strong Subjective, quite expensive, cumbersome to operate, not widely applicable

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