Adipose-derived stem cell preparation eye drops for treating corneal injury and preparation method thereof

An adipose stem cell and corneal injury technology, applied in the field of eye drops, can solve the problems of unsatisfactory visual function recovery, unsatisfactory tissue repair, obvious scar formation, etc., to achieve good corneal tissue repair effect, strong cell regeneration and repair , the effect is remarkable

Inactive Publication Date: 2017-11-07
成都远山博桥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: in the prior art, there are problems of unsatisfactory tissue repair, obvious scar formation, and unsatisfactory recovery of visual function. Adipose stem cell preparation eye drops and preparation method thereof

Method used

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  • Adipose-derived stem cell preparation eye drops for treating corneal injury and preparation method thereof
  • Adipose-derived stem cell preparation eye drops for treating corneal injury and preparation method thereof
  • Adipose-derived stem cell preparation eye drops for treating corneal injury and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] An adipose-derived stem cell preparation eye drop for treating corneal damage, comprising a cell culture solution, the cell culture solution is: the adipose-derived stem cells are first cultured in a serum-free medium for mesenchymal stem cells, and the serum-free medium for mesenchymal stem cells After the cell density in the medium reaches more than 90%, the culture supernatant is separated, and finally the culture supernatant is concentrated to obtain a concentrate.

[0030] The preparation method of above-mentioned cell culture fluid is specifically as follows:

[0031] Take 20ml of fat, wash it twice with normal saline, then add an equal volume of 0.25% type I collagenase for digestion, shake repeatedly during the digestion process, and after digesting for 60min, add an equal volume of primary culture medium to stop the digestion. After repeated blowing and mixing, the cells were washed with normal saline, and the supernatant was removed after centrifugation at 200...

Embodiment 2

[0034] The difference between this example and Example 1 is that the composition of the serum-free medium for mesenchymal stem cells is different, and the specific settings are as follows:

[0035] An adipose stem cell preparation eye drop for treating corneal damage, including cell culture solution, the preparation method of the above cell culture solution is: take 20ml of fat, wash it twice with normal saline, and then add an equal volume of 0.25% type I Collagenase was used for digestion, and the digestion process was shaken repeatedly. After digestion for 60 minutes, an equal volume of primary medium was added to stop the digestion. After repeated blowing and mixing, the cells were washed with normal saline, and the supernatant was removed after centrifugation at 2000rpm for 5 minutes. Add normal saline again to clean the cells, centrifuge at 2000rpm for 5 minutes, remove the supernatant, resuspend the pellet in a serum-free medium for mesenchymal stem cells, and transfer i...

Embodiment 3

[0038] This example is a comparative example of Example 2. The eye drops in this example consist of hrEGF with a final concentration of 1.5 μg / mL, bFGF with a final concentration of 0.4 μg / mL, and hLif with a final concentration of 0.4 μg / mL. composition.

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Abstract

The invention discloses adipose-derived stem cell preparation eye drops for treating corneal injury and a preparation method thereof and solves the problem that in the prior art, the tissue repair condition is less than satisfactory, cicatrization is obvious and visual function recovery is not ideal. The adipose-derived stem cell preparation eye drops comprise a cell culture liquid which is a concentrated liquid prepared by the following steps: culturing adipose-derived stem cells through a mesenchymal stem cell serum-free medium; when the cell density in the mesenchymal stem cell serum-free medium reaches 90% or above, performing separation to obtain a culture supernatant fluid; and finally concentrating the culture supernatant fluid to obtain the concentrated liquid. The adipose-derived stem cell preparation eye drops disclosed by the invention have the advantages of remarkably improving the cornea repair effect and the like.

Description

technical field [0001] The invention relates to an eye drop, in particular to an adipose stem cell preparation eye drop for treating corneal damage and a preparation method thereof. Background technique [0002] Eye health problems are closely related to the quality of vision. If the eyes are damaged, vision will be greatly affected. Corneal injury accounts for about 51% of serious eye injuries. The cornea is an important refractive medium. Different degrees of scars may be formed after corneal injury, or the curvature of the cornea may change, which seriously affects vision. Therefore, timely repair of corneal trauma can minimize the formation of scars and astigmatism to preserve and restore visual function. Common factors of corneal injury include: burns, trauma, infection and other factors. Common symptoms include tearing and pain in the eyes. If it is damaged by ultraviolet rays, there will be damage to the corneal epithelium, redness and swelling of the eyelids, conj...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/08A61P27/02
Inventor 杨远冯宇梁徐子林于洪涛李昕怡
Owner 成都远山博桥生物科技有限公司
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