Adipose-derived stem cell preparation eye drops for treating corneal injury and preparation method thereof
An adipose stem cell and corneal injury technology, applied in the field of eye drops, can solve the problems of unsatisfactory visual function recovery, unsatisfactory tissue repair, obvious scar formation, etc., to achieve good corneal tissue repair effect, strong cell regeneration and repair , the effect is remarkable
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Embodiment 1
[0029] An adipose-derived stem cell preparation eye drop for treating corneal damage, comprising a cell culture solution, the cell culture solution is: the adipose-derived stem cells are first cultured in a serum-free medium for mesenchymal stem cells, and the serum-free medium for mesenchymal stem cells After the cell density in the medium reaches more than 90%, the culture supernatant is separated, and finally the culture supernatant is concentrated to obtain a concentrate.
[0030] The preparation method of above-mentioned cell culture fluid is specifically as follows:
[0031] Take 20ml of fat, wash it twice with normal saline, then add an equal volume of 0.25% type I collagenase for digestion, shake repeatedly during the digestion process, and after digesting for 60min, add an equal volume of primary culture medium to stop the digestion. After repeated blowing and mixing, the cells were washed with normal saline, and the supernatant was removed after centrifugation at 200...
Embodiment 2
[0034] The difference between this example and Example 1 is that the composition of the serum-free medium for mesenchymal stem cells is different, and the specific settings are as follows:
[0035] An adipose stem cell preparation eye drop for treating corneal damage, including cell culture solution, the preparation method of the above cell culture solution is: take 20ml of fat, wash it twice with normal saline, and then add an equal volume of 0.25% type I Collagenase was used for digestion, and the digestion process was shaken repeatedly. After digestion for 60 minutes, an equal volume of primary medium was added to stop the digestion. After repeated blowing and mixing, the cells were washed with normal saline, and the supernatant was removed after centrifugation at 2000rpm for 5 minutes. Add normal saline again to clean the cells, centrifuge at 2000rpm for 5 minutes, remove the supernatant, resuspend the pellet in a serum-free medium for mesenchymal stem cells, and transfer i...
Embodiment 3
[0038] This example is a comparative example of Example 2. The eye drops in this example consist of hrEGF with a final concentration of 1.5 μg / mL, bFGF with a final concentration of 0.4 μg / mL, and hLif with a final concentration of 0.4 μg / mL. composition.
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