Method of inducing grass carp gynogenesis through fancy carp sperms and application gynogenesis grass carp

Abstract

The invention discloses a method of inducing grass carp gynogenesis through fancy carp sperms and application gynogenesis grass carp. The method includes following steps: (1), using Hank's liquid to dilute fancy carp seminal fluid, tiling in a culture dish, putting the culture dish on a shaker for centrifuging, and using an ultraviolet lamp to irradiate for deactivation; (2), mixing with mature grass carp first-class eggs, stirring, immediately putting into normal-temperature water for fertilization, and putting obtained fertilized eggs into cold water for cold treatment; (3), transferring into normal-temperature water for normal incubation, transferring into a cement tank for incubation, and putting fries with eyelids into a cement tank which is fertilized in advance for feeding. By utilizing sperms unique to fancy carp and innovation of the method, production efficiency of artificially induced gynogenesis grass carp can be improved remarkably, gynogenesis grass carp which is high in survival rate, growing speed and disease resistance is created on a large scale, and sufficient important germplasm resources are provided for large-scale development of disease-resistant grass carp.

Description

technical field [0001] The invention belongs to the field of fish variety improvement, and in particular relates to a method for inducing gynogenesis of grass carp by koi sperm and an application of gynogenesis of grass carp. Background technique [0002] Grass carp (Ctenopharyngodon idellus) is an excellent freshwater fish because of its herbivory, fast growth, and delicious meat. However, because grass carp is susceptible to diseases such as gill rot, saprolegniasis, hemorrhagic disease, fleas, enteritis, hepatobiliary syndrome and other diseases, the survival rate of grass carp is low, which seriously restricts the development of grass carp aquaculture. [0003] The applicant's laboratory has carried out research on fish gynogenesis for a long time, and found that fish eggs were stimulated to undergo gynogenesis with heterologous sperm, and the obtained gynogenetic offspring had chromosome fragments of heterologous sperm. This "hybridization" effect, The gynogenetic offs...

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Problems solved by technology

Koi has strong adaptability to water temperature, the optimum temperature range is 21-27°C, omnivorous, mainly molluscs, aquatic plants, benthic animals and fine algae, but up to now, no koi sperm has been used for female breeding. Related reports on nuclear development of grass carp

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