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Method for corylus heterophylla tissue culture

A Pingeu hybrid hazelnut and tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of unreasonable disinfection of a single stem section, difficult to successfully cultivate, and low survival rate of Pingeu hybrid hazel tissue. , to achieve the effect of less bacteria, easy disinfection and high survival rate

Inactive Publication Date: 2017-11-03
SHENYANG AGRI UNIV
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Problems solved by technology

[0004] In view of this, the present invention provides a method for tissue culture of Pingou hybrid hazel, which solves the problem of unreasonable medium and hormone ratio for tissue culture of Pingou hybrid hazel and disinfection of single stem segment for tissue culture, Then lead to the low survival rate of the Pingou hybrid hazel tissue, and the problem that the cultivation is not easy to succeed

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  • Method for corylus heterophylla tissue culture
  • Method for corylus heterophylla tissue culture
  • Method for corylus heterophylla tissue culture

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Embodiment 1

[0032] 1 Materials and methods

[0033] 1.1 Test material

[0034] The investigation of the varieties (lines) of the hazel resource nursery of Shenyang Agricultural University found that the varieties (lines) of Pingou hybrid hazel Dawei, Yuzhui, Liaozhen No. 3 and 84-69 not only have strong resistance, high yield and stable yield, but also have high yield Good quality, suitable for promotion in Shenyang and surrounding areas. In this experiment, the above varieties (lines) were selected as test materials, and the young shoots that had just germinated in late April, the vigorously growing shoots in mid-May, and the semi-lignified green branches in early June were taken as samples. Remove leaves and stipules from tender shoots, and cut new shoots and semi-lignified green shoots into 3-4 cm stem segments as explants for tissue culture.

[0035] Unless otherwise specified, in this test, 7g of agar per liter of culture medium was used, and other hormones were added according to ...

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Abstract

The invention relates to the technical field of tissue culture, and discloses a method for tissue culture of Pingou hybrid hazel: take the twigs of Pingou hybrid hazel as explants for tissue culture, disinfect with 70-75% alcohol for 10-30 seconds, Sterilize with 0.08-0.12% HgCl2 for 6-10 minutes, rinse and set aside; cut the above-mentioned stem section with buds into 1-2 cm long single-stem sections with buds, and inoculate them into the primary culture medium containing 7.5g / L PVP. First-generation culture; inoculate the single stem segment after the first-generation culture into the proliferation medium for proliferation culture, the proliferation medium is NRM medium, and the proliferation medium also contains 1.5mg / L of 6-BA and 0.01 mg / L of IBA; the stem segment after proliferation culture was dipped in 1g / L of NAA for 10s and then cut into the substrate. The method of the invention greatly improves the reproduction efficiency and survival rate of the Pingou hybrid hazel tissue culture seedlings.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of Pingou hybrid hazel. Background technique [0002] In production, hazelnut seedlings are mainly propagated by layering, and the layering technology is basically mature. However, the annual shoots of the roots of hazel trees are limited, so that the coefficient of layering propagation is relatively low, and the seedling speed is slow, which is far from meeting the large demand for hazel seedlings in production. When grafting and breeding, seedlings produce a large number of root tillers, which brings a lot of inconvenience to production and management, so it is seldom used. In recent years, although Chinese scientific research workers have made some progress in the cutting and tissue culture of cobs, the effect of application in production is not stable, so it is rarely used. Up to now, also do not find a kind of method that is simpler and m...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 董文轩赵万林胡爱双刘镇东
Owner SHENYANG AGRI UNIV
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