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Preparation method of vaccinium myrtillus extract

A technology for extracting and controlling flow rate, which is applied in the field of preparation of bilberry extract, can solve problems such as safety problems, and achieves the effects of easy operation, maintaining stability and simple process

Active Publication Date: 2017-10-24
杨凌萃健生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its described macroporous adsorption resin is self-made resin, there is safety problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Extraction for the first time: take 800g bilberry fruit, add 2.4L 95% ethanol solution and stir, then add 6.5g concentrated sulfuric acid, stir and extract at room temperature for 2h, add saturated sodium citrate solution 25g after extraction, adjust the pH of the solution to 3.0~3.5, stir for 15 minutes, centrifuge, collect the centrifugate, and filter the residue for later use;

[0045] (2) The second extraction: Put the filter residue after the first extraction and centrifugation into the extraction tank, add 3.6L 95% ethanol solution, start stirring, extract at room temperature for 1 hour, keep stirring during the extraction, centrifuge after the extraction, collect the filtrate, discard to filter residue;

[0046] (3) Pretreatment on the column: combine the two ethanol extraction centrifuges, concentrate until alcohol-free to obtain a concentrated solution, add water 3 times the volume of the concentrated solution and stir to obtain a feed solution, and prepare...

Embodiment 2

[0052] (1) The first extraction: take 1000g of lingonberry fruit, add 3L of 95% ethanol solution and stir, then add 7.5g of concentrated sulfuric acid, stir and extract at room temperature for 2h, add 30g of saturated sodium citrate solution after the extraction, and adjust the pH of the solution to 3.0 ~3.5, stir for 30 minutes, centrifuge, collect the centrifugate, and filter the residue for later use;

[0053] (2) The second extraction: Put the filter residue after the first extraction and centrifugation into the extraction tank, add 4.5L 95% ethanol solution, start stirring, extract at room temperature for 1 hour, keep stirring during the extraction, centrifuge after the extraction, collect the filtrate, discard to filter residue;

[0054] (3) Pretreatment on the column: combine the two ethanol extraction centrifuges, concentrate until alcohol-free to obtain a concentrated solution, add water 3 times the volume of the concentrated solution and stir to obtain a feed solutio...

Embodiment 3

[0060] (1) Extraction for the first time: take 900g bilberry fruit, add 2.8L 95% ethanol solution and stir, then add 7.0g concentrated sulfuric acid, stir and extract at room temperature for 2h, add saturated sodium citrate solution 27g after extraction, adjust the pH of the solution to 3.0~3.5, stir for 18 minutes, centrifuge, collect the centrifugate, and filter the residue for later use;

[0061] (2) The second extraction: Put the filter residue after the first extraction and centrifugation into the extraction tank, add 3.8L 95% ethanol solution, start stirring, extract at room temperature for 1 hour, keep stirring during the extraction, centrifuge after the extraction, collect the filtrate, discard to filter residue;

[0062] (3) Pretreatment on the column: combine the two ethanol extraction centrifuges, concentrate until alcohol-free to obtain a concentrated solution, add water 3 times the volume of the concentrated solution and stir to obtain a feed solution, and prepare...

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Abstract

The invention discloses a preparation method of a vaccinium myrtillus extract, which comprises the following steps of adding an acidized ethanol solution into fruits of vaccinium myrtillus, stirring and extracting, after the extracting is finished, stirring and adding a saturated sodium citrate solution, centrifuging, discarding filter residues, adding water into a concentrated solution and loading the concentrated solution on a macroporous adsorbent resin column, washing with a citric acid solution, desorbing by an ethanol solution, concentrating feed liquid until non-alcohol, and drying to obtain the vaccinium myrtillus extract. The preparation method is simple and convenient in process, easy to operate and convenient for industrialized production; the anthocyanin (accounting by cyanidin 3-0-glucoside chloride) content in the prepared vaccinium myrtillus extract is greater than or equal to 36%, and the total anthocyanin (accounting by cyanidin chloride) content is less than or equal to 1.0%, which accord with the requirements of United States pharmacopeia and European pharmacopoeia; the content of radionuclide <137>Cs in the vaccinium myrtillus extract is low, and is less than 10Bq / kg, which accord with the national standard of food safety (GB 14883.10-2016).

Description

technical field [0001] The invention relates to a plant extract, in particular to a preparation method of the bilberry extract. Background technique [0002] European lingonberry (Vaccinium myrtillus Linn.) is a kind of berry growing on perennial dwarf shrubs in Central and Northern Europe. Bilberry Extract is made from ripe bilberry berries and extracted into purple-black powder. It has the functions of preventing eye fatigue caused by video screens, relieving fatigue or leg swelling, preventing high blood pressure, and anti-aging. Into the European Pharmacopoeia (EP) Monographs on Herbal Drugs and Herbal drug preparations (Monographs on Herbal Drugs and Herbal drug preparations), and as a dietary supplement (Dietary Suppplments) in the United States Pharmacopoeia (USP). Because the anthocyanins contained in it have physiological activities such as anti-oxidation and scavenging free radicals, bilberry extract is mainly used in functional food and pharmaceutical industries....

Claims

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Application Information

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IPC IPC(8): A61K36/45A61P27/02A61P39/00A61P7/10A61P9/12A61P39/06A61K131/00
CPCA61K36/45A61K2236/333A61K2236/53A61K2236/55
Inventor 季亚飞周凯冯定军刘帮民梁岩
Owner 杨凌萃健生物工程技术有限公司
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