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Detection method of citrinin toxin in red yeast rice

A technology of red yeast rice and citrinin, which is applied in the field of detection, can solve the problems of complicated purification methods, and achieve the effects of simple extraction, high promotion and application value, and less time-consuming

Inactive Publication Date: 2017-10-20
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation and purification methods of citrinin toxin are relatively complicated. Generally, citrinin toxin is separated by concentration, column passing and extraction, and finally qualitative and quantitative detection is carried out by TLC or HPLC.

Method used

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  • Detection method of citrinin toxin in red yeast rice
  • Detection method of citrinin toxin in red yeast rice
  • Detection method of citrinin toxin in red yeast rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Citrinin toxin was extracted from red yeast rice sample A by two methods, and then quantitatively detected by HPLC. Specific steps are as follows:

[0037] 1. Utilize this method to extract citrinin toxin content in red yeast rice

[0038] Accurately weigh 1g of red yeast rice sample A that has been ground and sieved (passed through a 20-mesh sieve), put it in a 50mL centrifuge tube, add 5mL of chromatographic grade methanol, bathe in 65°C water for 60min, during this period, turn it upside down for 30s every 10min, and centrifuge at 8000rpm for 10min , stand still and take the supernatant into a 2mL micro-injection vial, use high-performance liquid chromatography (HPLC) to carry out high-performance liquid chromatography analysis on the sample liquid, and perform quantitative data processing with an external standard method. Red yeast rice sample A was repeated 3 times, and the average value was taken.

[0039] 2. Using immunoaffinity column as a control to extract c...

Embodiment 2

[0047] Embodiment 2, the detection of citrinin toxin in red yeast rice

[0048] Citrinin toxin was extracted from red yeast rice sample B by two methods, and then quantitatively detected by HPLC. Specific steps are as follows:

[0049] 1. Utilize this method to extract citrinin toxin content in red yeast rice

[0050] Accurately weigh 1g of red yeast rice sample B that has been fully mixed, put it into a 50mL centrifuge tube, add 5mL of chromatographic grade methanol, bathe in 65°C water for 60min, during this period, turn it upside down for 30s every 10min, centrifuge at 8000rpm for 10min, then let it stand and take the supernatant to In a 2mL micro-sampling vial, high-performance liquid chromatography (HPLC) was used to analyze the sample liquid by high-performance liquid chromatography, and the external standard method was used for quantitative data processing. Red yeast rice sample B was repeated 3 times, and the average value was taken.

[0051] 2. Using immunoaffinity...

Embodiment 3

[0060] Embodiment 3, the detection of citrinin toxin in red yeast rice

[0061] Citrinin toxin was extracted from red yeast rice sample C by two methods, and then quantitatively detected by HPLC. Specific steps are as follows:

[0062] 1. Utilize this method to extract citrinin toxin content in red yeast rice

[0063] Accurately weigh 1g of red yeast rice sample C that has been fully mixed, put it in a 50mL centrifuge tube, add 5mL of chromatographic grade methanol, and bathe in 65°C water bath for 60min. During this period, turn it upside down for 30s every 10min. In a 2mL micro-sampling vial, high-performance liquid chromatography (HPLC) was used to analyze the sample liquid by high-performance liquid chromatography, and the external standard method was used for quantitative data processing. Red yeast rice sample C was repeated 3 times, and the average value was taken.

[0064] 2. Using immunoaffinity column as a control to extract citrinin content in red yeast rice

[0...

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Abstract

The invention belongs to the field of detection, and provides a detection method of citrinin toxin in red yeast rice. The method comprises the following steps: 1) accurately weighing a red yeast rice sample, grinding and sieving with a 20-mesh sieve; 2) uniformly mixing the red yeast rice sample with methanol, reacting and extracting a citrinin toxin solution, wherein the mass volume ratio of the red yeast rice sample to the methanol is 1g to 5mL; 3) detecting the citrinin toxin solution extracted in the step 1) with high performance liquid chromatography. The invention provides a method for extracting the citrinin toxin from the red yeast rice, which is easier and more convenient, thereby promoting the development of the red yeast rice and the red yeast rice industry. As proved by testing, the method can be used for rapidly extracting the citrinin toxin from the red yeast rice, has the advantages of high efficiency, low time consumption, low cost and high recovery rate, and conforms to the laboratory quality control specification-food physical and chemical testing standard; moreover, the extraction of the citrinin toxin from the red yeast rice and relevant products thereof is simplified greatly, and the method has great popularization and application values.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a method for detecting citrinin toxin in red yeast rice. Background technique [0002] Citrinin is mainly a secondary metabolite produced by Penicillium, Aspergillus, and Monascus. Citrinin is widely distributed and mainly exists in grain products such as wheat, corn, oats, and processed fruits such as apples and pears. Citrinin has good antibacterial properties and can inhibit Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Streptomyces griseus, etc. It was used as a food preservative to smear on perishable meat in ancient my country. However, citrinin has not been widely used because of its strong nephrotoxicity, and its pollution has attracted more and more attention, and it has been listed as one of the toxins that must be detected by the European Branch of the Natural Toxin Detection Committee of the International Academy of Life Sciences. [0003] China is th...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘阳关绚丽
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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