Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for quickly detecting zearalenone toxin in vegetable oil

A technology of zearalenone and vegetable oil, which is applied in the field of detection, achieves the effects of high promotion and application value, less time-consuming, and simple detection

Inactive Publication Date: 2017-10-20
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clear standard for the detection of zearalenone toxin in vegetable oil, and zearalenone toxin in edible oil has become an important factor affecting food safety in my country. Can it be quickly and accurately detected in corn oil? The content of erythraralenone toxin has also become the main content of the inspection and quarantine of edible oil export in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly detecting zearalenone toxin in vegetable oil
  • Method for quickly detecting zearalenone toxin in vegetable oil
  • Method for quickly detecting zearalenone toxin in vegetable oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The test strips and immunoaffinity column were used to detect zearalenone toxin in vegetable oil samples (commercially available corn oil). Specific steps are as follows:

[0028] 1. Using test strips to detect the content of zearalenone toxin in corn oil samples

[0029] Accurately measure 1g of corn oil sample that has been fully mixed with a pipette, put it in a 50mL centrifuge tube, add 9mL of 70% analytical grade methanol (a solution prepared with analytical grade methanol), vortex at room temperature for 2min, centrifuge at 10000rpm for 5min, and statically Put 100 μL of the supernatant into a 2 mL centrifuge tube, add 1 mL of dilution buffer, mix by inverting up and down for 20 seconds, and then use a test strip to detect the toxin content. The corn oil samples were repeated 3 times and the average value was taken.

[0030] 2. Detection of zearalenone toxin content in corn oil samples by immunoaffinity column

[0031] Use a pipette to accurately measure 4g of ...

Embodiment 2

[0038] The test strips and immunoaffinity column were used to detect zearalenone toxin in vegetable oil samples (commercially available corn oil). The parameters different from Example 1 are:

[0039] Use a pipette to accurately measure 1 g of the fully mixed corn oil sample, place it in a 50 mL centrifuge tube, add 1 mL, 2 mL, and 5 mL of 70% analytical grade methanol respectively, and use a test strip to detect the concentration of the vegetable oil sample (commercially available corn oil). Zearalenone toxin. Each sample was performed three times in parallel.

[0040] The test results showed that the average recoveries of corn oil samples and 70% analytical grade methanol, 1:1, 2:1, 5:1 (mass volume ratio g:mL) were 35.01%, 48.20%, 61.26%, respectively.

[0041] Accurately measure 1g of corn oil sample that has been fully mixed with a pipette, put it in a 50mL centrifuge tube, add 5mL of 70% analytical grade methanol and acetonitrile respectively, and use a test strip to d...

Embodiment 3

[0044] The test strips and immunoaffinity column were used to detect zearalenone toxin in vegetable oil samples (commercially available corn oil). The parameters different from Example 1 are:

[0045] Use a pipette to accurately measure 1g of corn oil sample that has been fully mixed, put it in a 50mL centrifuge tube, add 9mL of 100% and 9mL of 85% analytical grade methanol solution (prepared with analytical grade methanol), and use a test strip to detect the vegetable oil sample (commercially available corn oil) zearalenone toxin. Each sample was performed three times in parallel.

[0046] The test results showed that the average recoveries of corn oil samples and 100% and 85% methanol were 72.14% and 68.23%, respectively.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for quickly detecting zearalenone toxin in vegetable oil. The method comprises the steps of uniformly mixing the vegetable oil and a methanol solution, centrifuging or carrying out settling separation after reaction, getting a supernatant, and utilizing a zearalenone toxin test strip for detecting. According to the detection method provided by the invention, the physicochemical property that zearalenone is easy to dissolve in organic solvents such as acetonitrile, methyl alcohol, ethyl alcohol, ethyl acetate and acetone is utilized, so that the method for simply and conveniently detecting zearalenone toxin in the vegetable oil is invented, and the development of oil and vegetable oil industries is promoted. The tests show that the method provided by the invention can be used for quickly detecting the zearalenone toxin in the vegetable oil, is high in efficiency, less in consuming time, low in cost and high in recovery rate, and meets the laboratory quality control standard-food physical and chemical detection standard.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a detection method of zearalenone toxin. Background technique [0002] Zearalenone (Zearaleuoue, ZEN), also known as F-2 toxin, is a non-steroidal mycotoxin mainly produced by Fusarium graminearum (Fusarium gramiuearum) and yellow Fusarium (F. culmorum). ZEN has strong reproductive toxicity and teratogenic effects. Zearalenone was first isolated from scab corn. It is widely found in moldy corn, sorghum, wheat, oats, barley and other grains and dairy products. It is the most widely polluted Fusarium toxin in the world. After zearalenone contaminates feed and food, it not only seriously affects the agricultural economy, but also brings serious food safety problems and threatens human health. Due to the harm of zearalenone to human body and animals, many countries have established the limit standard of mitaralenone in food. Australia stipulates that the mass fractio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/75G01N30/02
CPCG01N21/75G01N30/02
Inventor 刘阳关绚丽
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com