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Preparation of a gold magnetic nanoparticle and its combination with surface-enhanced Raman spectroscopy for rapid detection of tetrodotoxin

A technology of gold magnetic nanoparticles and tetrodotoxin, applied in Raman scattering, measuring devices, and material analysis through optical means, can solve the problems of lack of specificity, poor repeatability, and low sensitivity

Active Publication Date: 2020-07-28
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the mouse biological method can accurately and stably detect tetrodotoxin, and is a legal method for determining TTX content in Japan, it is time-consuming and laborious, with poor repeatability and lack of specificity
However, liquid chromatography is mostly used for the quantitative detection of tetrodotoxin, but it requires complex and time-consuming pretreatment of samples before detection, which is not suitable for rapid on-site detection.
Thin-layer chromatography is simple to operate, but the sensitivity is too low for quantitative detection and analysis
The antiserum preparation method in the immunological method is called simple, but because the specificity of the monoclonal antibody is poor, there may be cross-reaction, so it will have a great impact on the quantitative detection of tetrodotoxin

Method used

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  • Preparation of a gold magnetic nanoparticle and its combination with surface-enhanced Raman spectroscopy for rapid detection of tetrodotoxin
  • Preparation of a gold magnetic nanoparticle and its combination with surface-enhanced Raman spectroscopy for rapid detection of tetrodotoxin
  • Preparation of a gold magnetic nanoparticle and its combination with surface-enhanced Raman spectroscopy for rapid detection of tetrodotoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] i) Surface Band-NH 2 and Fe of -SH 3 o 4 / SiO 2 Preparation of nanoparticles

[0050] Take 4.00mg Fe 3 o 4 Nanoparticles were washed twice with distilled water, collected by magnetic separation after washing, dispersed with 2mL distilled water, put into a 15mL glass bottle, and 11mL absolute ethanol and 0.2mL ammonia water were added at the same time. Sonicate for 10 minutes under the condition of pH=11, add 8 μL TEOS, and react under ultrasound for 8 hours. After the reaction, the particles are collected by magnetic separation, washed twice with absolute ethanol and distilled water, and finally mixed with 2 mL of distilled water and 11 mL of absolute ethanol. Disperse in the mixed liquid, add 0.2mL ammonia water, sonicate for 10min, then add 5.3μL APTES and 5.3μL MPTES, and react for 18h under the action of sonication. After the reaction, the particles were collected by magnetic separation to obtain the surface band -NH 2 and Fe of -SH 3 o 4 Nanoparticles were...

Embodiment 2

[0057] (1) Surface Tape-NH 2 and Fe of -SH 3 o 4 / SiO 2 Preparation of nanoparticles

[0058] Take 4.00mg Fe 3 o 4 Nanoparticles were washed twice with distilled water, collected by magnetic separation after washing, dispersed with 2mL distilled water, put into a 15mL glass bottle, and added 9mL absolute ethanol and 0.2mL ammonia water at the same time. Sonicate for 5 minutes under the condition of pH = 10, add 8 μL TEOS, and react under ultrasound for 6 hours. After the reaction, the particles are collected by magnetic separation, washed twice with absolute ethanol and distilled water, and finally mixed with 2 mL of distilled water and 9 mL of absolute ethanol. Disperse in the mixed solution, add 0.2mL ammonia water, sonicate for 5min, then add 3.2μL APTES and 3.2μL MPTES, and react for 16h under the action of sonication. After the reaction, the particles were collected by magnetic separation to obtain the surface band -NH 2 and Fe of -SH 3 o4 Nanoparticles were washe...

Embodiment 3

[0066] (A) Only modify -NH 2 Fe 3 o 4 / SiO 2 magnetic nanoparticles

[0067] Take 4.00mg Fe 3 o 4 Nanoparticles were washed twice with distilled water, collected by magnetic separation after washing, dispersed with 2mL distilled water, put into a 15mL glass bottle, and 10mL absolute ethanol and 0.2mL ammonia water were added at the same time. Sonicate for 5 minutes under the condition of pH = 10, add 8 μL TEOS, and react for 6 hours under ultrasound. After the reaction, the particles are collected by magnetic separation and magnetic separation, washed twice with absolute ethanol and distilled water, and finally dispersed with 2 mL and 10 mL of absolute ethanol , add 0.2mL ammonia water, sonicate for 5min, then add 8μL APTES, and react for 16h under the action of sonication. After the reaction, the particles were collected by magnetic separation to obtain the surface band -NH 2 Fe 3 o 4 Nanoparticles were washed twice with absolute ethanol and distilled water, and fina...

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Abstract

The invention provides a surface enhanced raman spectrum based quick tetrodotoxin (TTX)detection method of tetrodotoxin (TTX). A nano Fe3O4 particle is adopted as a magnetic substrate; Fe3O4 is modified in sequence by using tetraethoxysilane (TEOS), 3-aminopropyltriethoxysilane and 3-mercaptopropyltriethoxysilane in sequence; a gold nano particle is adsorbed on a modified magnetic nano particle through the electrostatic adsorption of -NH2 and the action of a Au-S bond; a well assembled gold magnetic nano particle is integrally modified by using the TEOS and the 3-aminopropyltriethoxysilane; an immunocompetent gold magnetic nano particle is used as a raman substrate; RhB-ITC (Rhodamine B-Isothiocyanate) / anti-TTX is used as a raman signaling molecule; the quick detection is carried out on the TTX by utilizing a surface enhanced raman spectrometer; the detection limit can reach 0.01[mu]g / ml in under the condition that the gold magnetic nano particle is concentrated by an outer magnet; the detection linear range is 0.01[mu]g / mL to 0.5[mu]g / mL; the recovery rate of a detected sample is 82.64 to 92.60 percent; the relative standard deviation is 2.6 to 11.1 percent. The detection of the TTX has important significance in the aspects of food safety and medical science.

Description

technical field [0001] The invention relates to a preparation method of gold magnetic nanoparticles and its application in surface-enhanced Raman spectrum rapid detection of tetrodotoxin. Background technique [0002] Tetrodotoxin (TTX) is an alkaloid contained in puffer fish (commonly known as puffer fish) and other organisms. Its molecular formula is C 11 h 17 o 8 N 3 , with a molecular weight of 319. It is an amino perhydroquinazoline compound, one of the most toxic neurotoxins found in nature, and was once considered to be the most toxic non-protein toxin in nature. 0.5mg can cause death. The chemical and thermal properties of tetrodotoxin are very stable, and can only be decomposed by heating at high temperature for more than 30 minutes or under alkaline conditions. [0003] At present, the detection methods of tetrodotoxin mainly include mouse biological method, liquid chromatography, thin layer chromatography, immunological method and so on. Although the mouse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 能静孙培龙项晨陆嘉晖王栩俊郏侃
Owner ZHEJIANG UNIV OF TECH
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