Preparation method of tuna extract with inhibitory effect on cancer cells

Abstract

The invention discloses a preparation method of tuna extract with inhibitory effect on cancer cells, and preparation steps include compound enzyme enzymolysis, isoelectric point precipitation, ultrafiltration, nanofiltration, HPLC elution and freezing drying. The beneficial effects are that tuna protein has a lot of marine Brevibacterium oxydan secreting alkaline protease and undariapinnatifida protease cleavage sites, after the enzymolysis, lots of different lengths of peptide chains can be obtained, and the enzymolysis efficiency is high; the preparation method has the advantages of being simple, less in equipment, low in preparation cost, accurate in operation data of each step, good in repeatability, and suitable for mass production; the purity of the prepared bioactive short peptide is high, DNA double helix structure unwinding of the cancer cells can be effectively inhibited by the short peptides, so that the DNA replication of the cancer cells can be inhibited, the cancer cells stay in interphase of cell division, secretion of E-cadherin can be promoted, moveability of the cancer cells is reduced, and the transfer speed of the cancer cells is reduced.

Description

technical field [0001] The invention relates to the technical field of preparation of health care products, in particular to a preparation method of tuna extract which has the function of inhibiting cancer cells. Background technique [0002] As we all know, malignant tumor has become a major disease that threatens human health, and its treatment has also become one of the hot spots that people pay attention to. Traditional chemotherapy and radiotherapy also produce serious side effects during treatment. Tumor biotherapy is the fourth treatment after traditional therapy. Current research has confirmed that biotherapy is playing an increasingly important role in the treatment of tumors and is expected to become a therapy for overcoming tumors. [0003] Peptides have only been paid attention to since the beginning of the 20th century. Studies have found that they are different from proteins and amino acids, and have the effect of drugs. It was not until the 1990s that there ...

AI Technical Summary

Problems solved by technology

Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. The above method uses protease extracted from land to enzymatically hydrolyze marine organisms, and the enzymatic hydrolysis efficiency is low.