Chromatographic column based on graphene and zinc oxide double-layer coating stationary phase and preparation method for stationary phase thereof

Abstract

The invention provides a chromatographic column based on a graphene and zinc oxide double-layer coating stationary phase and a preparation method for the stationary phase thereof and belongs to the technical field of chromatographic separation. According to the invention, a zinc oxide film is prepared into a rough transition layer, so that the rough transition layer can be used as a transition layer between an inner wall of the chromatographic column and a graphene material; the action of chemical bonding and mechanical anchoring is utilized to promote the adhesive force of the nanometer graphene material to the inner wall of the chromatographic column and promote the stability and uniformity of the stationary phase; the problems of difficulty in coating on the inner wall of the chromatographic column and poor stability of the nanometer graphene slice material can be solved; the defect of no universality of the traditional self-assembling method is overcome; the method is suitable for the graphene, graphene oxide and reduction-oxidation graphene materials to form the stationary phase on the inner wall of the chromatographic column; besides, the method has the advantages of simple steps, low cost, uniform dispersion and firm adhesion of the prepared products and is beneficial to the large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of chromatographic separation, and in particular relates to a chromatographic column based on a graphene-based and zinc oxide double-layer film stationary phase and a preparation method for the stationary phase thereof. Background technique [0002] Chromatographic columns play an important role in chromatographic technology, and samples are separated through chromatographic columns. However, the current commercial chromatographic columns are still difficult to separate certain mixtures, especially compounds with similar boiling points and positional isomers with similar structures. As we all know, the separation principle of the chromatographic column is to use the different distribution coefficients of the sample components on the chromatographic stationary phase to achieve the purpose of analytical separation. Therefore, as the core part of the chromatographic column, the selection of the chromatographic...

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Problems solved by technology

[0007] However, the coupling agent method in the prior art not only cannot prepare reduced graphene oxide (RGO), but also prepares graphene (G) because graphene oxide (GO) needs to be prepared first and then reduced to graphene, so the steps are cumbersome and costly. lack of time

Since the self-assembly method uses electrostatic attraction, it requires the material to have sufficient charge, which is relatively easy to meet for the preparation of graphene oxide (GO), but graphene cannot achieve electrostatic self-assembly in principle; in addition, the reduction of the charge of graphene oxide Compared with graphene oxide, the electric quantity is greatly reduced, which increases the difficulty of preparation, reduces the adhesion of the film, and makes it difficult for the stationary phase to withstand the multiple washings of the mobile phase during chromatographic separation.

In summary, the existing methods for preparing graphene-based stationary phases cannot be fully applied to the three graphene materials of graphene, graphene oxide and reduced graphene, which has led to the application of graphene-based nanomaterials in this field. Therefore, it is urgent to develop a new method for the preparation of graphene-based stationary phases

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