Preparation method of hierarchical drug-loaded cornea repairing material

A technology of loading drugs and repairing materials, applied in the field of biomedicine, can solve the problems of difficult repair, long time and slow metabolic process.

Inactive Publication Date: 2017-10-20
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no blood vessels in the cornea, and the nutrition is mainly supplied by the limbal vascular netwo

Method used

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  • Preparation method of hierarchical drug-loaded cornea repairing material
  • Preparation method of hierarchical drug-loaded cornea repairing material
  • Preparation method of hierarchical drug-loaded cornea repairing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 0.8g of gelatin, add it to 80g of hydrochloric acid solution (1wt%), stir mechanically for 4h, stirring speed is 800r / min, fully dissolve to obtain 1wt% (weight ratio) gelatin solution; inject 2mL of gelatin solution into the mold and spread it flat At the bottom, a gelatin film was obtained after vacuum drying for 2 hours at an ambient temperature of 35°C. Add vancomycin to deionized water to prepare a drug solution with a concentration of 1mg / mL, add 0.5mL of the drug solution dropwise on the surface of the dried gelatin film and spread it evenly, place it at a temperature of 35°C, and dry it in vacuum for 1h After the drug completely infiltrates the film and dries, add the gelatin solution and the drug solution in an alternate cycle and repeat the above steps. Finally, a layered drug-loaded corneal repair material with a thickness of 200 μm was obtained. With the same preparation process and conditions, corneal repair materials that are dried naturally at room...

Embodiment 2

[0024] Weigh 0.6g of collagen, add it to 60g of weak acetic acid solution (1.5wt%), mechanically stir for 48h, stirring speed is 100r / min; inject 2mL of collagen solution into the mold and spread it on the bottom, at an ambient temperature of 37°C The collagen film was prepared after vacuum drying for 6 h. Add tobramycin to deionized water to prepare a drug solution with a concentration of 1mg / mL, add 0.5mL of the drug solution dropwise on the surface of the dried gelatin film and spread it evenly, place it at a temperature of 37°C, and dry it in vacuum 4 hours after the drug completely infiltrates the film and dries, add the collagen solution and the drug solution alternately and repeatedly, and repeat the above steps. Finally, a layered drug-loaded corneal repair material with a thickness of 400 μm was obtained. Under the same preparation conditions and the amount of raw materials, due to the interaction between tobramycin drug and collagen molecular chains, the mechanical ...

Embodiment 3

[0026] Take by weighing 0.9g chitosan, join in 30g concentration and be 1wt% hydrochloric acid solution, mechanically stir 4h, stirring speed 800r / min, fully dissolve and make 30wt% (weight ratio) chitosan solution; Inject 1.5mL collagen solution In the mold, the chitosan film was obtained after vacuum drying for 5 hours at an ambient temperature of 35°C. Add ceftriaxone to deionized water to prepare a drug solution with a concentration of 1mg / ml, drop 0.45ml of the drug solution on the surface of the dried chitosan film and spread it evenly, place it at a temperature of 35°C, and vacuum After drying for 3 hours, after the drug was completely infiltrated into the film and dried, the chitosan solution and the drug solution were repeatedly added alternately, and the above steps were repeated; finally, a layered drug-loaded corneal repair material with a thickness of 300 μm was obtained. When the temperature is too high, some drugs are easily inactivated. In the normal temperatur...

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Abstract

The invention discloses a preparation method of a hierarchical drug-loaded cornea repairing material. The preparation method comprises the following steps: firstly, adding a polymer material into a solvent to prepare a polymer solution; secondly, drying the polymer solution to form a film; thirdly, dropwise adding a drug solution on the surface of a polymer film and then carrying out uniform tiling on the drug solution. According to the preparation process disclosed by the invention, the hierarchical drug-loaded cornea repairing material has the advantages of simple and feasible technology and good repeatability, and is suitable for mass production of novel functional cornea repairing materials.

Description

technical field [0001] The invention adopts the methods of preparing the corneal repair material first and then loading the drug and during the process of preparing the corneal repair material, and relates to the field of biomedicine. Background technique [0002] The cornea is located in the front of the eyeball and forms the outer wall of the eyeball together with the sclera. Its texture is transparent and it is an important part of the refractive medium. It is the first line of defense to protect the eyes from external factors. There are no blood vessels in the cornea, and the nutrition is mainly supplied by the limbal vascular network, and the metabolic process is slow; so once a lesion occurs, it is difficult and takes a long time to repair. Corneal disease is a common and frequently-occurring disease in ophthalmology, and it is also one of the main causes of blindness. There are more than 10 million blind patients in my country due to corneal diseases, 80% of whom can...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/24A61L27/22A61L27/20A61L27/54A61L27/50
Inventor 任力
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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