Method of tracing places of origin of aquatic products based on amino acid carbon stable isotopes

Abstract

The invention discloses a method of tracing places of origin of aquatic products based on amino acid carbon stable isotopes; the method comprises the steps of subjecting a pretreated sample to amino acid extraction before purification; adding hydrochloric acid into the sample, hydrolyzing, centrifuging, subjecting supernate to purification of strong cation exchange columns, concentrating the purified liquid via nitrogen blowing process to obtain sample amino acid; adding amino acids into thionyl chloride-isopropanol solution by means of N-pivaloyl and O-isopropanol ester (NPP) process, performing amino acid derivation, blow-drying by nitrogen, acylating by pivaloyl chloride, passing through silica gel chromatographic columns, concentrating the obtained liquid by a nitrogen blowing process to obtain a derivative, dissolving in ethyl acetate for cryopreservation, and performing amino acid C stable isotope measurement, to be specific, analyzing and identifying an amino acid ester sample via a gas chromatograph-mass spectrometer; comparing amino acid delta 13C values of samples from different sea areas to identify different types of aquatics from different sea areas.

Description

technical field [0001] The invention belongs to the field of food safety, and relates to a method for tracing the origin of aquatic products based on amino acid carbon stable isotopes, which is mainly used for identifying and tracing the real origin of aquatic products. Background technique [0002] my country is a big aquaculture country, and its aquaculture production accounts for more than 80% of the world's total production, ranking first in the world. However, food safety issues have been hindering the development and upgrading of the industry. The construction of the aquatic product quality traceability system will help solve the problem of adulteration and sale of aquatic products, and can effectively protect the vital interests of aquatic product manufacturers, farmers and consumers. In recent years, my country has successively implemented identification management in the quality traceability of aquatic products from breeding, processing to circulation, etc., and ap...

AI Technical Summary

Problems solved by technology

However, food safety issues have been hindering the development and upgrading of the industry

For the stable isotope detection technology of the whole sample, because the technology selects the whole muscle sample, it will be affected by many factors, such as the different material composition of different growth stages of organisms, the difference in the absorption and transformation efficiency of different compounds, and isotope fractionation. Therefore, this technology has certain limitations, making the identification results not accurate enough and not convincing.

The content of characteristic compounds such as fatty acids, carbohydrates, and amino acids is affected by their feeding, and has different content characteristics. For the origin traceability technology of aquatic products, there is also the application of characteristic compound composition content analysis technology. According to the differences in the characteristic compound composition content of samples from different regions The origin of aquatic products is identified, but the individual differences in the content of biological characteristic compounds are obvious, and there are significant differences with seasonal changes, which will also make the identification results inaccurate

Images