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Extraction method of wild antheraea pernyi silk fibroin

A technology of tussah silk fibroin protein and extraction method, which is applied in the direction of animal/human peptides, peptide sources, peptides, etc., can solve the problems of incomplete extraction of silk protein, waste of materials, failure to extract silk protein, etc., and achieve non-toxic and harmless extraction Ability, simple extraction process, and environmental protection of the extraction method

Inactive Publication Date: 2017-09-29
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing methods often use strong acid, strong alkali, high-concentration salt solution such as high-concentration CaCl 2 Solution, cuproammonia solution, lithium bromide solution, lithium thiocyanide solution, etc. dissolve tussah silk fibroin, but the extraction of silk protein by the above method is often not thorough, and the silk protein in the silkworm cocoon cannot be completely extracted, resulting in Material waste, so it is necessary to develop a method that can extract silk protein from silkworm cocoons as completely as possible to improve the utilization rate of raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0024] A) Weigh 10 g of tussah silk and add it to deionized water with a bath ratio of 1:50, adjust the pH to 2.5 with hydrochloric acid, degumming in a water bath at 95°C for 60 minutes, and add NaHCO after the water bath is over 3 Adjust the pH to neutral and repeat the operation 3 times. The degummed tussah silk was soaked in 200 mL deionized water for 2 h, and then dried at 60 °C.

[0025] B) Weigh 10 g FeCl 2 , add 50 mL of deionized water to dissolve, then add 15 mL of tetrasodium iminodisuccinate dropwise, mix well and dilute to 100 mL.

[0026] C) Weigh 1 g of the dried silk sample obtained in step A), add 15 mL of the mixed solution prepared in step B), and keep stirring on a 60°C water bath for 60 min.

[0027] D) After taking out and waiting for cooling, place the solution under 30 kHz ultrasonic wave for 30 min, and at the same time slowly adjust the pH to 8.5 with acetic acid, and adjust the pH of the solution to neutral after the ultrasonic treatment.

[0028]...

Embodiment approach 2

[0030] A) Weigh 10 g of tussah silk and add it to deionized water at a bath ratio of 1:50, adjust the pH to 2.7 with hydrochloric acid, degumming in a water bath at 95°C for 60 minutes, and add NaHCO after the water bath is over 3 Adjust the pH to neutral and repeat the operation 3 times. The degummed tussah silk was soaked in 200 mL deionized water for 2 h, and then dried at 60 °C.

[0031] B) Weigh 10 g FeCl 2 , add 50 mL of deionized water to dissolve, then add 15 mL of tetrasodium iminodisuccinate dropwise, mix well and dilute to 100 mL.

[0032] C) Weigh 1 g of the dried silk sample obtained in step A), add 15 mL of the mixed solution prepared in step B), and keep stirring on a 60°C water bath for 60 min.

[0033] D) After taking out and waiting for cooling, place the solution under 40kHz ultrasonic wave for 30 minutes, and at the same time adjust the pH to 8.8 slowly with acetic acid, and adjust the pH of the solution to neutral after the ultrasonic treatment.

[0034...

Embodiment approach 3

[0036] A) Weigh 10 g of tussah silk and add it to deionized water with a bath ratio of 1:50, adjust the pH to 3 with hydrochloric acid, degumming in a water bath at 95°C for 60 minutes, and add NaHCO after the water bath is over 3 Adjust the pH to neutral and repeat the operation 3 times. The degummed tussah silk was soaked in 200 mL deionized water for 2 h, and then dried at 60 °C.

[0037] B) Weigh 10 g FeCl 2 , add 50 mL of deionized water to dissolve, then add 15 mL of tetrasodium iminodisuccinate dropwise, mix well and dilute to 100 mL.

[0038] C) Weigh 1 g of the dried silk sample obtained in step A), add 15 mL of the mixed solution prepared in step B), and keep stirring on a 60°C water bath for 60 min.

[0039] D) After taking out and cooling down, place the solution under 50 kHz ultrasonic wave for 30 minutes, and at the same time slowly adjust the pH to 9 with acetic acid, and adjust the pH of the solution to neutral after the ultrasonic treatment.

[0040] E) The...

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Abstract

The invention relates to the field of silk fibroin extraction, and discloses an extraction method of wild antheraea pernyi silk fibroin. The extraction method comprises the following steps: degumming and drying tussah silks; extracting the wild antheraea pernyi silk fibroin with a mixed solution system of FeCl2 and tetrasodium iminodisuccinate, and adjusting pH under ultrasonic assistance in an extracting process in order to increase the extraction rate; performing ultrafiltration on a solution undergoing ultrasonic treatment, performing freeze drying on the remaining solution after ultrafiltration, and grinding the obtained wild antheraea pernyi silk fibroin into powder for later use. The method provided by the invention is easy to operate, and has low environmental harm and high protein extraction rate; the obtained silk fibroin has higher purity.

Description

technical field [0001] The invention relates to the field of silk fibroin extraction, in particular to a method for extracting tussah silk fibroin. Background technique [0002] Tussah silk is mainly composed of silk fibroin and sericin, among which the content of silk fibroin is about 70%, which is the main component of silk. Sericin and silk fibroin have different structures and properties: sericin is a globular protein, and the constituent amino acids contain a large number of polar hydrophilic side groups, which have poor stability and are soluble in hot water, acid, alkali, protease and low-concentration sodium carbonate, etc. Silk fibroin is a fibrous protein, natural silk fiber contains a large number of intramolecular and intermolecular hydrogen bonds, and has a high degree of crystallinity and is difficult to dissolve in general solvents. Sericin surrounds the surface layer of silk fiber and acts as an adhesive. Sericin and silk fibroin have different structures...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14C07K1/34
CPCC07K14/43586
Inventor 王秉李津梁军龙陈茹茹何宇杰
Owner ZHEJIANG SCI-TECH UNIV
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