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A kind of wild plantain in vitro culture multiplication method

An in vitro culture, wild banana technology, applied in the direction of plant regeneration, horticultural methods, botanical equipment and methods, etc., to achieve the effect of obvious effect, not easy to pollute, and rapid proliferation

Active Publication Date: 2019-09-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, these two kinds of proliferation and differentiation media have not been reported on the in vitro culture of wild plantain

Method used

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  • A kind of wild plantain in vitro culture multiplication method
  • A kind of wild plantain in vitro culture multiplication method
  • A kind of wild plantain in vitro culture multiplication method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation before vaccination.

[0023] The inoculation knife, tweezers, filter paper, and inoculation tray used for inoculation were autoclaved for later use. Put the high-pressure inoculation knife, tweezers, filter paper, inoculation tray, culture medium, alcohol lamp, lighter, marker pen, industrial alcohol and waste liquid tank into the ultra-clean workbench, and sterilize with ultraviolet light for 20 minutes. Proliferation medium and differentiation medium were screened for concentration of growth regulators using single factor four-level or three-level experiments. The screening media are: L1: MS+6-BA 1.0 mg / L; L2: MS+6-BA 2.5 mg / L; L3: MS+6-BA 4.0 mg / L; L4: MS+6-BA 5.5 mg / L. L5: MS+NAA 1.0 mg / L; L6: MS+NAA 2.0 mg / L; L7: MS+NAA 3.0 mg / L. Sucrose 30g / liter, agar 6g / liter, pH adjusted to 5.8, 30 bottles of each medium were prepared, and the high pressure was used for later use.

[0024] 2. Vaccination.

[0025] Wipe your hands and arms with 75% alcohol, d...

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Abstract

The present invention provides a method for in-vitro culture proliferation of wild banana, and belongs to the field of in-vitro culture proliferation of a plant tissue. A special block-shape tissue in the in-vitro culture process of the wild banana is used as a material for culture by a proliferation medium and a differentiation medium, the proliferation rate is fast, the operation is simple, and pollution is not easy to cause. In short, the special block-shape tissue in the tissue culture process of the wild banana and the proliferation medium and the differentiation medium can control effectively the growth state of the special block-shape tissue, and are used for seedling proliferation, differentiation and germplasm resource in-vitro preservation of Sanming wild banana. Compared with a traditional medium, the proliferation medium and the differentiation medium are more convenient, and only one growth regulator is used. The method has the advantages of simple operation, low cost, high proliferation rate and low pollution rate, and the mediums have broad prospects.

Description

technical field [0001] The invention relates to an in vitro culture and multiplication method of wild banana, which belongs to the field of plant tissue culture in vitro multiplication, and is mainly used in in vitro multiplication of Sanming wild banana, in vitro preservation of germplasm resources and transgene. Background technique [0002] Compared with cultivated bananas, wild bananas have a large number of stress-resistant genes. Studies have found that wild bananas in Sanming have important stress-resistant genes such as cold resistance and disease resistance. They are valuable germplasm resources for basic research and important research in banana bioengineering research. Material. In the traditional banana in vitro propagation technology, the sucking buds of bananas are generally selected as explants to establish an aseptic system for in vitro propagation, and then the sucking buds are induced to proliferate on the proliferation medium, but the proliferation speed o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘炜婳林争春赖钟雄温陈金
Owner FUJIAN AGRI & FORESTRY UNIV
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