Paraffin section method for pomegranate seed particles

Abstract

The invention discloses a paraffin section method for pomegranate seed particles. The paraffin section method comprises the following steps: (1) material selection and fixing; (2) dehydration and transparency: carrying out gradient dehydration, carrying out treatment for 2 h in each gradient, and then converting into absolute ethyl alcohol treatment for 1.5 h twice; adding a mixed solution of absolute ethyl alcohol and xylene in a volume ratio of 2: 1 and a mixed solution of absolute ethyl alcohol and xylene in a volume ratio of 1: 2 for 2 h, and then converting into pure xylene treatment for 1.5 h twice; (3) waxing and embedding: putting the transparent materials into a mixed solution of xylene and paraffin in a volume ratio of 1: 1 for waxing for 4 to 5 h, then putting in a mixed solution of xylene and paraffin in a volume ratio of 1: 2 for waxing for 4 to 5 h, then waxing for 4 to 5 h with pure paraffin, and then continuously waxing for 3 to 4 d by changing the pure paraffin; (4) section and spreading; and (5) dyeing to be transparent, and mounting. A section tissue manufactured by the method is complete in structure and clear in cell boundary, and the sections are clean and easy to observe.

Description

technical field [0001] The invention relates to the technical field of paraffin sectioning of plants, in particular to a method for paraffin sectioning pomegranate grains. Background technique [0002] Pomegranate (Punica granatum) is a deciduous shrub or small tree of the genus Punica in the Lythraceae family. It is native to Iran and Afghanistan. . Pomegranate fruit is a round or oval fruit wrapped in a leathery peel. There are 5-7 ventricles inside the peel, and each ventricle is composed of dozens to hundreds of seeds. The seeds are the edible parts of the fruit, which is different from apples, Pears, strawberries, peaches, plums, apricots, and longan are all different. The pomegranate seed coat is composed of two layers of inner and outer seed coats. The outer seed coat is thick and juicy, rich in sugar, organic acids, polyphenols, etc., and is the main edible part; the inner seed coat is rich in lignin, cellulose, hemicellulose, etc. It is the main factor of grain h...

AI Technical Summary

Problems solved by technology

However, due to the large structure difference between the inner and outer testa of pomegranate, the outer testa is thick and juicy, and the inner testa is rich in lignin, cellulose, hemicellulose, etc., and the leather is hard. It is difficult to obtain a complete and clear cut by traditional paraffin section method. Microsection brings certain challenges to the observation of pomegranate seed coat structure

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