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Novel method for producing biodiesel from rice straw

A technology of rice straw and biodiesel, which is applied in biofuel, fermentation and other directions, can solve the problems of secondary environmental pollution, shortage of arable land, affecting the yield and quality of biodiesel, etc., and achieves low production cost, reduced raw material cost, and alleviates the environment. The effect of pollution

Inactive Publication Date: 2017-09-26
金丹凤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The currently widely used esterification method to prepare biodiesel requires not only large-scale planting of oil crops, but also various physical and chemical treatments for oil crops. Excessive methanol and free fatty acids used in the reaction process will seriously affect the yield and quality of biodiesel. , the discharge of waste alkali and acid is likely to cause secondary pollution to the environment
Moreover, our country is not a big country for growing oil crops, and the land is small, the population is large, and the arable land is scarce, so it is impossible to grow oil crops on a large scale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A new method for producing biodiesel from rice stalks, comprising the steps of:

[0016] S1, Trichoderma, Aspergillus oryzae and Phanerochaete chrysosporium were mixed in a ratio of 90:8:2, rice straw was used as substrate, 28°C, no humidity control, closed static fermentation for 4 days, and rice straw hydrolyzate was obtained ;

[0017] S2. Using rice straw hydrolyzate as a substrate, using Trichosporium fermentum to carry out liquid state fermentation, fermenting at 25° C. for 8 days, with a pH of 6.5, to obtain microbial oil with polyunsaturated fatty acids as components.

[0018] S3. Using the Escherichia coli homologous recombinant bacterium with the ability to synthesize fatty acid ethyl ester as the fermentation strain, using the rice straw hydrolyzate prepared in S1 as the sugar solution and the microbial oil obtained in S2 as the fatty acid source to prepare biodiesel. The raw material is 4% of rice straw hydrolyzate (sugar content), 1% of microbial oil, 1% o...

Embodiment 2

[0020] A new method for producing biodiesel from rice stalks, comprising the steps of:

[0021] S1, Trichoderma, Aspergillus oryzae and Phanerochaete chrysosporium were mixed in a ratio of 90:8:2, rice straw was used as substrate, 30°C, no humidity control, closed static fermentation for 4 days, and rice straw hydrolyzate was obtained ;

[0022] S2. Using rice straw hydrolyzate as a substrate, using Trichosporium fermentum to carry out liquid state fermentation, fermenting at 26° C. for 8 days, with a pH of 6.5, to obtain microbial oil composed of polyunsaturated fatty acids.

[0023] S3. Using the Escherichia coli homologous recombinant bacterium with the ability to synthesize fatty acid ethyl ester as the fermentation strain, using the rice straw hydrolyzate prepared in S1 as the sugar solution and the microbial oil obtained in S2 as the fatty acid source to prepare biodiesel. The raw material is 4% of rice straw hydrolyzate (sugar content), 1% of microbial oil, 1% of pepto...

Embodiment 3

[0025] A new method for producing biodiesel from rice stalks, comprising the steps of:

[0026] S1, Trichoderma, Aspergillus oryzae and Phanerochaete chrysosporium were mixed in a ratio of 90:8:2, rice straw was used as substrate, 29°C, no humidity control, closed static fermentation for 4 days, to obtain rice straw hydrolyzate ;

[0027] S2. Using rice straw hydrolyzate as a substrate, using Trichosporium fermentum to carry out liquid state fermentation, fermenting at 28° C. for 8 days, with a pH of 6.5, to obtain microbial oil composed of polyunsaturated fatty acids.

[0028] S3. Using the Escherichia coli homologous recombinant bacterium with the ability to synthesize fatty acid ethyl ester as the fermentation strain, using the rice straw hydrolyzate prepared in S1 as the sugar solution and the microbial oil obtained in S2 as the fatty acid source to prepare biodiesel. The raw material is 4% of rice straw hydrolyzate (sugar content), 1% of microbial oil, 1% of peptone, 0.5...

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Abstract

The invention discloses a novel method for producing biodiesel from rice straw. The novel method includes steps of mixing trichoderma, aspergillus oryzae and phanerochaete chrysosporium with one another according to a proportion of 90:8:2 and carrying out solid-state fermentation; carrying out uncontrolled wet closed static fermentation for 4 days and converting the rice straw into diversified small-molecule carbohydrates; producing polyunsaturated fatty acid from straw hydrolysate by means of fermentation by the aid of fermentation trichosporon asahii at the temperatures of 25-28 DEG C; preparing the biodiesel from prepared rice straw hydrolysate and prepared unsaturated fatty acid by the aid of constructed Escherichia coli homologous recombinant bacteria with fatty acid ethyl ester (namely, biodiesel) synthesis ability. The straw hydrolysate is used as a raw material for the polyunsaturated fatty acid. The rice straw hydrolysate is used as sugar liquor for the biodiesel, and the unsaturated fatty acid is used as a raw material for the biodiesel. The novel method has the advantages that as shown by gas chromatographic analysis, the biodiesel prepared by means of fermentation mainly comprises ethyl oleate, ethyl myristate, ethyl palmitate and ethyl 9-hexadecenoate; various indexes of the biodiesel produced by the aid of the novel method conform to international biodiesel standards.

Description

technical field [0001] The invention relates to the field of new energy, in particular to a new method for producing biodiesel from rice straw. Background technique [0002] Energy is the basis of all life. Currently, the energy consumed by our lives is mainly fossil energy. However, the exploitation and utilization of fossil energy is not a long-term solution. my country's oil, coal, power supply shortages and other energy issues have become increasingly prominent, and energy shortages have become a bottleneck restricting my country's economic development. Therefore, the development of economically feasible and environmentally friendly new energy sources is imminent for the future development of mankind. [0003] The currently widely used esterification method to prepare biodiesel requires not only large-scale planting of oil crops, but also various physical and chemical treatments for oil crops. Excessive methanol and free fatty acids used in the reaction process will ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/649Y02E50/10
Inventor 金丹凤
Owner 金丹凤
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