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Method for determination of free radical over-oxidative capacity of environment

A measurement method and technology of free radicals, applied in the direction of material electrochemical variables, etc., can solve the problems of high cost of use, less monitoring and research of environmental persistent free radicals, and restrictions on the research, monitoring and governance of environmental persistent free radicals

Inactive Publication Date: 2017-09-22
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Electron paramagnetic resonance has the disadvantages of expensive equipment, high cost of use, and harsh detection conditions. It is difficult to popularize, and it cannot be measured on the spot in a timely manner. It also lacks an evaluation method for free radical peroxidation ability, which restricts the research on environmental persistent free radicals. Monitoring and Governance
The monitoring and research of environmental persistent free radicals are seldom carried out in China

Method used

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  • Method for determination of free radical over-oxidative capacity of environment
  • Method for determination of free radical over-oxidative capacity of environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Coating carbon nano-materials on the screen-printed electrodes and drying to prepare carbon nano-based electrodes; dripping 4.5 μL of lecithin with a concentration of 10 mg / mL and 4.5 μL of cholesteryl cyclohexane with a concentration of 5 mg / mL on the surface of the above-mentioned carbon nano-based electrodes Alkane, a vacuum desiccator protected from light for 1h, after drying, place it in 0.1mol / LKCl solution, scan the -0.2V potential range to be stable by cyclic voltammetry, and place it in a vacuum bag after drying, vacuum, and store at 4℃; Add the eluent to the tested contaminated soil, and place it in the sun to irradiate the reaction, stop the light, take the supernatant and place it in the colorimetric tube, and add 2mL of NaH with a concentration of 0.75mol / L 2 PO 4 And 2mL Na with a concentration of 0.75mol / L 2 HPO 4 Buffer; put in the carbon nano-based electrode, incubate the electrode unit at a constant temperature at 37.5°C for 20 minutes to peroxidize the l...

Embodiment 2

[0029] The carbon nano-materials are coated on the screen-printed electrodes and dried to prepare the carbon nano-based electrodes; on the surface of the carbon nano-based electrodes, 5.5 μL of lecithin at a concentration of 10 mg / mL and 5.5 μL of cholesteryl cyclohexane at a concentration of 5 mg / mL are dripped Alkane, in a light-proof vacuum dryer for 1.5h, after drying, place it in 0.1mol / LKCl solution, scan the 1.0V potential range to be stable by cyclic voltammetry, and place it in a vacuum bag after drying, vacuum, and store at 4℃; Add the eluent to the tested contaminated soil, and place it under the sun to irradiate the reaction, stop the light, take the supernatant and put it in the colorimetric tube, and add 3mL NaH with a concentration of 0.75mol / L 2 PO 4 And 3mL Na with a concentration of 0.75mol / L 2 HPO 4 Buffer; put in the carbon nano-based electrode, incubate the electrode unit at a constant temperature of 37.5°C for 30 minutes to peroxidize the lipid of the electr...

Embodiment 3

[0031] The carbon nano-materials are coated on the screen-printed electrodes and dried to prepare carbon nano-based electrodes; 5 μL of lecithin with a concentration of 10 mg / mL and 5 μL of cholesterol cyclohexane with a concentration of 5 mg / mL are dripped on the surface of the above-mentioned carbon nano-based electrodes. Avoid light in a vacuum desiccator for 1.2h. After drying, place it in 0.1mol / LKCl solution, and scan the 0.5V potential range to be stable by cyclic voltammetry. After drying, place it in a vacuum bag, vacuum, and store at 4℃; Try adding the eluent to the contaminated soil, and place it under the sun to irradiate the reaction, stop the light, take the supernatant and put it in a colorimetric tube, and add 2.5mL NaH with a concentration of 0.75mol / L 2 PO 4 And 2.5mL Na with a concentration of 0.75mol / L 2 HPO 4 Buffer; put in the carbon nano-based electrode, incubate with a stopper at 37.5 ℃ constant temperature to make the electrode unit double-layer phospholi...

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Abstract

The invention provides a method of manufacturing electrochemical sensor array for evaluation of the free radical over-oxidative capacity of the environment. The method overcomes the shortage in the prior art, provides an electro-chemical sensor array for measuring free radical lipid over-oxidation of the environment and a method which is quick and easy, low in cost, easy to popularize, field applicable and persistent for evaluating the free-radical over-oxidation capacity and durability of the environment. The novelty of the method is that, although the bilayer phospholipid membrane bio-membrane sensor is currently the most active frontier field in biomedicine, the biomimetic membrane electrochemical sensor array and electrochemical method used for the determination of the free radical over-oxidative capacity in field environment cannot make any discoveries.

Description

Technical field [0001] The invention relates to a method for manufacturing an electrochemical sensor array for evaluating the peroxidation ability of environmental free radicals. Background technique [0002] Environmental persistent free radicals are a new type of environmental risk substances with high reactivity. Biological organisms are highly hazardous. EPFRs are proposed relative to the traditionally concerned short-lived free radicals, which refer to organic substances that exist in the environment for tens of minutes to several hours, have paramagnetic stability, and can induce more oxidative stress in biological systems. The transition metal element iron is rich in tropical and subtropical soils, and the region has a long sunshine time and strong ultraviolet rays, which are easy to stimulate to produce EPFR. Some scholars have observed that phenolic substances adsorbed in red soil can produce EPFRs with a lifespan of up to 5 days under sunlight. They may dissociate fro...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/30
Inventor 高云涛速敏熊华斌杨志李晓芬郭秋爽施志凡
Owner YUNNAN MINZU UNIV
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