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Method for semi continuous fermentation of L-alanine

A semi-continuous, alanine technology, applied in the field of L-alanine semi-continuous fermentation, can solve the problems of waste of energy consumption, low output and the like, and achieve the effects of low energy consumption, high output and simple process

Inactive Publication Date: 2017-09-15
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of waste of energy consumption and low output in the prior art, the present invention provides a method for semi-continuous fermentation of L-alanine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for semi-continuous fermentation of L-alanine, comprising the steps of:

[0034] (1) Inoculate shaker flask seeds into 800L seed tanks, inject sterile compressed air 1:1vvm, 37°C, cultivate for 6 hours, OD600 value is 35, and obtain seed liquid;

[0035] (2) Combine 120g / L glucose with component A: 10g / L yeast extract powder, 8g / L peptone, 0.25g / L potassium dihydrogen phosphate, 0.6g / L magnesium sulfate, 2g / L ammonium phosphate, 0.05g / L bubble enemy, sterilize at 120~122°C respectively, cool to below 60°C, add glucose to component A to obtain culture medium:

[0036] (3) Sterilize a 5t fermenter at 120-122°C for 20 minutes with saturated water vapor, add 3.6t of sterilized medium, 400L of seed liquid, adjust the pH to 6.8 with ammonia water, and inject sterile compressed air 1:1.0vvm , 35°C, cultured with stirring at 150rpm for 7h, when the OD value reached 12, stop the aeration, continue stirring at 50rpm for 20h, discharge the fermentation broth with 25% of...

Embodiment 2

[0039] A method for semi-continuous fermentation of L-alanine, comprising the steps of:

[0040] (1) Inoculate the shake flask seeds into an 800L seed tank, inject sterile compressed air 1:1.5vvm, and culture at 38°C for 7 hours, with an OD600 value of 40 to obtain a seed solution;

[0041] (2) Combine 180g / L glucose with component A: 8g / L yeast extract powder, 5g / L peptone, 0.5g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate, 1.5g / L ammonium phosphate, 0.1 g / L foam enemy, sterilized at 120~122°C respectively, cooled to below 60°C, added glucose to component A to obtain the culture medium:

[0042] (3) Sterilize a 5t fermenter with saturated water vapor at 120-122°C for 23 minutes, add 3.6t of sterilized medium, 180L of seed liquid, adjust the pH to 7 with ammonia water, and inject sterile compressed air 1:0.7vvm , 36°C, cultured with stirring at 200rpm for 4h, when the OD value reached 10, stop aeration, and continue to stir at 100rpm. After 16h, discharge the fe...

Embodiment 3

[0045] A method for semi-continuous fermentation of L-alanine, comprising the steps of:

[0046] (1) Inoculate the shake flask seeds into an 800L seed tank, inject sterile compressed air 1: 0.4vvm, 35°C, cultivate for 4 hours, and the OD600 value is 30 to obtain the seed solution;

[0047] (2) Combine 150g / L glucose with component A: 5g / L yeast extract powder, 10g / L peptone, 0.3g / L potassium dihydrogen phosphate, 0.2g / L magnesium sulfate, 2g / L ammonium phosphate, 0.06g / L bubble enemy, sterilize at 120~122°C respectively, cool to below 60°C, add glucose to component A to obtain culture medium:

[0048](3) Sterilize a 5t fermenter with saturated water vapor at 120-122°C for 25 minutes, add 3.6t of sterilized medium, 720L of seed liquid, adjust the pH to 7.2 with sulfuric acid, and inject sterile compressed air 1:0.5vvm , 38°C, cultured with stirring at 180rpm for 5h, when the OD value reached 15, stop the aeration, continue to stir at 80rpm for 18h, discharge the fermentation ...

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Abstract

The invention relates to the technical field of fermentation engineering, and in particular relates to a method for semi continuous fermentation of L-alanine. The method comprises the following steps: preparation of a seed fluid; preparation of a culture medium; semi continuous fermentation culture of the L-alanine; collection of fermentation broth; and purification to obtain the L-alanine. The method is simple in process, green and environmentally-friendly, eliminates the step of disinfection of each batch of the seed liquid, a fermentation tank and the culture medium, and has the advantages of saving time, low energy consumption and high yield.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a method for semi-continuous fermentation of L-alanine. Background technique [0002] L-alanine is colorless to white crystalline powder, soluble in water and ethanol, insoluble in ether and acetone. It is mainly used in biochemical research, tissue culture, liver function test, flavor enhancer, can increase the seasoning effect of condiments, and can also be used as a sour taste corrector to improve the sour taste of organic acids. [0003] However, the existing L-alanine fermentation method mostly adopts the method of batch fermentation, and each batch of seed liquid, fermenter and culture medium needs to be sterilized separately, resulting in waste of energy consumption and low yield . Contents of the invention [0004] Aiming at the problems of waste of energy consumption and low yield in the prior art, the invention provides a method for semi-continuous ...

Claims

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Application Information

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IPC IPC(8): C12P13/06
CPCC12P13/06
Inventor 李斌水张仲良张习伟李霆李敬敬
Owner JING JING PHARMA
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