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Method for extracting isoflavones from soybean molasses

A technology of soybean isoflavones and soybean molasses, applied in the direction of organic chemistry, etc., can solve the problems of low purity and yield of soybean isoflavones, cumbersome and complicated processes, etc., and achieve the effects of green environmental protection, energy consumption, high purity, and improved utilization rate

Active Publication Date: 2017-09-15
山东中阳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The Chinese patent application number 201210517707.0 uses soybeans as raw materials for its production method, and obtains soybean isoflavone products through drying, peeling, grinding, beating, heating, and ethanol extraction. Germ or soybean meal is used as raw material, extracted with methanol, flocculated, and finally purified by column chromatography to obtain soybean isoflavone products with a purity higher than 50%. There are also some pre-treatments in the preparation process, such as crushing and extraction, and finally purified with resin ; Simultaneously the soybean isoflavone product purity and productive rate that above-mentioned method prepares are all lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The described method utilizing soybean molasses to extract soybean isoflavones comprises the following steps:

[0029] 1) Pretreatment: adjust the sugar content of soybean molasses to 15% with distilled water, insert 2g / L yeast after sterilization, and collect ethanol after fermentation at 25°C for 48 hours to obtain soybean molasses fermentation liquid;

[0030] 2) Deproteinization and reducing sugar treatment: Boil the soybean molasses fermentation liquid for 10 minutes, then add 1‰ of the total mass of soybean molasses fermentation liquid CaCO 3 Stir, continue to add 1% CaO of the total mass of the soybean molasses fermentation liquid, stir evenly and then stand still, stir once every 5 minutes until the liquid level is layered, and then pass CO into the solution. 2 , adjust the pH of the solution to 7, and centrifuge 10min under the condition of 3000r / min at the centrifuge speed, get the supernatant, wherein the protein removal rate is 99.3%, the reducing sugar remo...

Embodiment 2

[0037] The described method utilizing soybean molasses to extract soybean isoflavones comprises the following steps:

[0038] 1) Pretreatment: adjust the sugar content of soybean molasses to 25% with distilled water, insert 8g / L yeast after sterilization, and collect ethanol after fermentation at 35°C for 48 hours to obtain soybean molasses fermentation liquid;

[0039] 2) Deproteinization and reducing sugar treatment: Boil the soybean molasses fermentation liquid for 15 minutes, then add 2‰ of the total mass of the soybean molasses fermentation liquid CaCO 3 Stir, continue to add 4% CaO of the total mass of the soybean molasses fermentation liquid, stir evenly and then stand still, stir once every 10 minutes until the liquid level is layered, and then feed CO into the solution. 2 , adjust the pH of the solution to 7.5, and centrifuge 20min under the condition of 8000r / min at the centrifuge speed, get the supernatant, wherein the protein removal rate is 99.5%, the reducing sug...

Embodiment 3

[0046] The described method utilizing soybean molasses to extract soybean isoflavones comprises the following steps:

[0047]1) Pretreatment: adjust the sugar content of soybean molasses to 20% with distilled water, insert 6g / L yeast after sterilization, ferment at 30°C for 48 hours, collect ethanol, and obtain soybean molasses fermentation liquid;

[0048] 2) Deproteinization and reducing sugar treatment: Boil the soybean molasses fermentation liquid for 12 minutes, then add 1.5‰ of the total mass of the soybean molasses fermentation liquid with CaCO 3 Stir, continue to add 2% CaO of the total mass of the soybean molasses fermentation liquid, stir evenly and then stand still, stir once every 8 minutes until the liquid level is layered, and then feed CO into the solution. 2 , adjust the pH of the solution to 7.2, and centrifuge 15min under the condition of 5000r / min at the centrifuge speed, get the supernatant, wherein the protein removal rate is 99.6%, the reducing sugar remo...

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PUM

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Abstract

The invention discloses a method for extracting isoflavones from soybean molasses, belonging to the technical field of production of soybean products. The method provided by the invention overcomes the problems of complex process, low purity of extracted isoflavones and low extraction rate in the prior art. The method comprises the following steps: diluting soybean molasses with distilled water, then carrying out fermentation with microzymes, boiling soybean molasses fermentation broth, adding CaCO3 and CaO, carrying out uniform mixing under stirring, then carrying out standing, adjusting the pH value of the obtained solution to 7, carrying out centrifugation and taking supernatant; subjecting the supernatant to heating and column chromatography; and then separately eluting resin by using ethanol solutions with mass fractions of 0 to 20% and 30 to 40% so as to obtain an ethanol solution of isoflavones, carrying out distillation to recover ethanol, adding ethyl acetate into the obtained concentrate of isoflavones, successively carrying out stirring, standing and settling, subjecting the obtained supernatant to concentration so as to obtain isoflavone paste, and drying and crushing the isoflavone paste. The method provided by the invention is applicable to extraction of isoflavones from soybean molasses.

Description

technical field [0001] The invention relates to a method for extracting soybean isoflavones from soybean molasses, belonging to the technical field of soybean product production. Background technique [0002] Soybean molasses is a by-product produced in the process of preparing soybean protein concentrate from defatted soybean meal by alcohol method. It is rich in biologically active substances. Soybean isoflavone is one of them, and its application value is extremely high. Soy isoflavones can lower blood cholesterol, prevent osteoporosis and inhibit the growth of cancer cells. It can also be used as a substitute for estrogen therapy drugs to improve women's menopausal syndrome. At present, soybean molasses is mostly used to prepare feed, and the main raw material for extracting soybean isoflavones is soybean meal or directly soybean, which forms a double waste, which not only wastes the biologically active substances in soybean molasses, but also affects the production of s...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 管勇佳魏艳姜自强杜现祥安玉辉张玉琛
Owner 山东中阳生物科技有限公司
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