Processing method of protein peptide product from low-value fishes
A processing method and technology of low-value fish, which is applied in the field of processing low-value fish protein peptide products, can solve problems such as unreasonable composition of compound marine fish protein peptide powder, unsatisfactory functional effects, and large molecular weight of protein peptide powder. Achieve the effect of not easy to oxidize and deteriorate, improve the therapeutic effect, and have high nutritional value
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Embodiment 1
[0019] A processing method for a low-value fish protein peptide product, comprising the following steps:
[0020] 1) Pretreatment: Wash the low-value fish, remove the head, tail and viscera, scrape off the scales on the surface, remove the bones, remove the spines and leave the fish meat, and beat;
[0021] 2) Degreasing: Add phospholipase A1 and phospholipase A2 to the fish homogenate for enzymatic hydrolysis. The amounts of phospholipase A1 and phospholipase A2 added are 0.1-0.4% and 0.3-0.7% of the volume of the fish homogenate, respectively. The enzymolysis temperature is 35~45℃, the pH is 8~9, and the enzymolysis time is 2~3h. After enzymatic hydrolysis, the enzyme is inactivated, and the fat is removed by supercritical carbon dioxide extraction. The extraction pressure is 10~25Mpa, the carbon dioxide flow rate is 30~100L / h, the extraction temperature is 40~55℃, and the extraction time is 4~6h. Phospholipase A1 and phospholipase A2 enzymatically decompose the fat in the...
Embodiment 2
[0029] A processing method for a low-value fish protein peptide product, comprising the following steps:
[0030] 1) Pretreatment: Wash the low-value fish, remove the head, tail and viscera, scrape off the scales on the surface, remove the bones, remove the spines and leave the fish meat, and beat;
[0031] 2) Degreasing: Add phospholipase A1 and phospholipase A2 to the fish homogenate for enzymatic hydrolysis. The amounts of phospholipase A1 and phospholipase A2 added are 0.3% and 0.6% of the volume of the fish homogenate, respectively. The enzymolysis temperature is 40°C, the pH is 8.5, and the enzymolysis time is 2.7h. After enzymatic hydrolysis, the enzyme is inactivated, and the fat is removed by supercritical carbon dioxide extraction. The extraction pressure is 18Mpa, the carbon dioxide flow rate is 60L / h, the extraction temperature is 50°C, and the extraction time is 5h. Phospholipase A1 and phospholipase A2 enzymatically decompose the fat in the fish homogenate into...
Embodiment 3
[0039] A processing method for a low-value fish protein peptide product, comprising the following steps:
[0040] 1) Pretreatment: Wash the low-value fish, remove the head, tail and viscera, scrape off the scales on the surface, remove the bones, remove the spines and leave the fish meat, and beat;
[0041] 2) Degreasing: Add phospholipase A1 and phospholipase A2 to the fish homogenate for enzymatic hydrolysis. The amounts of phospholipase A1 and phospholipase A2 added are 0.4% and 0.7% of the volume of the fish homogenate, respectively. The enzymolysis temperature is 40°C, the pH is 8.5, and the enzymolysis time is 2.3h. After enzymatic hydrolysis, the enzyme is inactivated, and the fat is removed by supercritical carbon dioxide extraction. The extraction pressure is 21Mpa, the flow rate of carbon dioxide is 40L / h, the extraction temperature is 50°C, and the extraction time is 5h. Phospholipase A1 and phospholipase A2 enzymatically decompose the fat in the fish homogenate i...
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