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Amino acid and acyl carnitine dry blood spot control material and preparing method thereof

A technology of acylcarnitine and amino acid, which is applied in the field of amino acid and acylcarnitine dried blood spot quality control products and its preparation, and can solve the problems of high product price, no stable source, and different characteristics

Inactive Publication Date: 2017-09-08
GUANGZHOU KINGMED DIAGNOSTICS CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the variety of compounds detected by tandem mass spectrometry at one time, in general, in order to ensure its quality control effect, it is also necessary to add a variety of components to its quality control products, and because of the different characteristics of each component, it is difficult to prepare a uniform and stable quality control product. Control
Therefore, at present, there are few sources of commercial amino acid and acylcarnitine dry blood spot quality control products, and the products are expensive, the delivery cycle is long, and there is no stable source for the same batch number

Method used

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  • Amino acid and acyl carnitine dry blood spot control material and preparing method thereof
  • Amino acid and acyl carnitine dry blood spot control material and preparing method thereof
  • Amino acid and acyl carnitine dry blood spot control material and preparing method thereof

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preparation example Construction

[0037] A method for preparing the above-mentioned amino acid and acylcarnitine dried blood spot quality control product, comprising the steps of:

[0038] 1) Preparation of erythrocyte fluid: collect healthy human whole blood, separate red blood cells, adjust the hematocrit to a predetermined value with normal saline, obtain erythrocyte fluid, and measure the background value of each amino acid and acylcarnitine;

[0039] 2) Preparation of standard solution: using an aqueous solvent to dissolve amino acids and / or water-soluble acylcarnitines; using alcohol solvents to dissolve fat-soluble acylcarnitines to obtain amino acid standard solutions and / or acylcarnitine standard solutions;

[0040] 3) Preparation of blood spot quality control: Add amino acid standard solution and / or acylcarnitine standard solution to the set value to the above red blood cell solution to obtain a working solution; take the above working solution dropwise on the carrier, dry to obtain amino acid and acy...

Embodiment 1

[0057] 1) Separation of red blood cells:

[0058] Collect healthy human heparin anticoagulated whole blood, requiring no obvious hemolysis, jaundice, fat or pollution, centrifuge at a speed of 3000r / min for 5min, separate the supernatant and white membrane after centrifugation, add about the same amount of normal saline as the amount of red blood cells , use a mixer to mix well for 5-7min, centrifuge at 3000r / min for 5min, separate the supernatant and buffy membrane, repeat this step 3 times to obtain red blood cells. Normal saline was used to adjust the hematocrit to 50% to obtain erythrocyte fluid, and the background concentrations of amino acids and acylcarnitine in the erythrocyte fluid were determined.

[0059] 2) Preparation of amino acids and acylcarnitines:

[0060] Use physiological saline to dissolve 8 amino acid standards including alanine Ala, phenylalanine Phe, leucine Lue, methionine Met, valine Val, citrulline Cit, arginine Arg, and tyrosine Tyr product, to ob...

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Abstract

The invention discloses an amino acid and acyl carnitine dry blood spot control material and a preparing method thereof. The dry blood spot control material is prepared from eight kinds of amino acid standard substances, 15 kinds of acyl carnitine standard substances and erythrocytes. According to the amino acid and acyl carnitine dry blood spot control material, the use quantity of standard substances is greatly reduced, the stability and homogeneity meet the requirement, and the amino acid and acyl carnitine dry blood spot control material can be stored for 4 months or longer at minus 20 DEG C. The preparing method is low in cost and easy to implement, the amino acid and acyl carnitine dry blood spot control material can be prepared in a large amount and is long in usage cycle, quality-control batch numbers do not need to be changed frequently, daily quality-control analysis is convenient, and thus the demand for the control material in clinical and daily detection is met.

Description

technical field [0001] The invention relates to a quality control product for detection and a preparation method thereof, in particular to an amino acid and acylcarnitine dried blood spot quality control product and a preparation method thereof. Background technique [0002] Inherited metabolic disease refers to the functional defects of synthesized enzymes, receptors, carriers and other proteins due to gene mutations, resulting in various abnormalities in the synthesis, metabolism, transport and storage of biochemical substances in the body. It belongs to a part of monogenic genetic diseases, most of which are autosomal recessive inheritance, a few are autosomal dominant inheritance, X-linked sex-linked inheritance, or mitochondrial inheritance; there are many kinds of genetic metabolic diseases, 500-600 kinds are common, The total number may reach more than thousands of species; the incidence rate of single disease is low, but the overall incidence rate is relatively high,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 陈秀如佘旭辉赵蓓蓓程雅婷韦兰清李卓阳倪周吴婉琼邓珍容陈静宜欧婷
Owner GUANGZHOU KINGMED DIAGNOSTICS CENT
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