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Method for extracting microalgal oil

A technology of microalgae oil and extraction method, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of environmental pollution, high price, high energy consumption, etc., achieve high economic and environmental benefits, reduce post-processing costs, and prepare The effect of simple method

Inactive Publication Date: 2017-09-05
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most common applications are imidazole and pyridine ionic liquids. The main raw materials of such ionic liquids, such as alkyl-substituted imidazoles and alkyl-substituted pyridines, are derived from petroleum, and most of them are volatile organic compounds and are expensive.
The preparation methods of ionic liquids include direct synthesis method, indirect synthesis method, microwave synthesis method, ultrasonic synthesis method and electrochemical synthesis method. material, and high energy consumption
Studies have shown that ionic liquids are toxic and poorly biodegradable
The above shortcomings severely limit the wide application of ionic liquids in the pretreatment of microalgae cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare the deep eutectic solvent: mix choline chloride and urea uniformly at a molar ratio of 1:1, heat and stir at 90°C for 2 hours to obtain a colorless transparent liquid, and cool to room temperature to obtain the deep eutectic solvent.

[0027] The extraction of microalgae oil is carried out according to the following steps:

[0028] (1) Pretreatment: According to the dosage ratio of 5mL deep eutectic solvent: 1g chlorella algae powder, mix the prepared deep eutectic solvent and chlorella algae powder evenly, and stir magnetically at room temperature 23°C-28°C The mixed solution was left for 24 hours, and then the mixed solution was centrifuged at 10000 rpm for 10 minutes, and the lower solid phase was removed. Then wash the lower solid phase with deionized water for 3 times, centrifuge the washing mixture at a speed of 6000rpm for 10min after each washing, and then freeze-dry the washed solid phase in a freeze dryer at -80°C for 48h to obtain Pre-treated chlorel...

Embodiment 2

[0034] Prepare the deep eutectic solvent: mix choline chloride and glycerin uniformly at a molar ratio of 1:2, heat and stir at a constant temperature of 80°C for 2 hours to obtain a colorless transparent liquid, and obtain the deep eutectic solvent after cooling to room temperature.

[0035] The extraction of microalgae oil is carried out according to the following steps:

[0036] (1) Pretreatment: According to the dosage ratio of 25mL deep eutectic solvent: 1g chlorococcus algae powder, mix the prepared deep eutectic solvent and chlorococcus algae powder evenly, and stir magnetically at room temperature 23℃~28℃ The mixed solution was left for 24 hours, and then the mixed solution was centrifuged at 10000 rpm for 10 minutes, and the lower solid phase was removed. Then wash the lower solid phase with deionized water for 3 times, centrifuge the washing mixture at a speed of 6000rpm for 10min after each washing, and then freeze-dry the washed solid phase in a freeze dryer at -80°C...

Embodiment 3

[0042] Preparation of deep eutectic solvent: mix choline chloride and oxalic acid uniformly at a molar ratio of 1:3, heat and stir at a constant temperature of 70°C for 2 hours to obtain a colorless transparent liquid, and obtain a deep eutectic solvent after cooling to room temperature.

[0043] The extraction of microalgae oil is carried out according to the following steps:

[0044] (1) Pretreatment: According to the dosage ratio of 40mL deep eutectic solvent: 1g Scenedesmus powder, mix the prepared deep eutectic solvent and Scenedesmus powder evenly, and magnetically stir the mixture at a room temperature of 23°C to 28°C After 72 hours, the mixture was centrifuged at 10,000 rpm for 10 minutes, and the lower solid phase was removed. Then wash the lower solid phase with deionized water for 3 times, centrifuge the washing mixture at a speed of 6000rpm for 10min after each washing, and then freeze-dry the washed solid phase in a freeze dryer at -80°C for 48h to obtain Pretrea...

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Abstract

The invention relates to a method for extracting microalgal oil. The extraction method comprises the following steps: (1) performing pretreatment, namely uniformly mixing a eutectic solvent with microalgae powder, stirring the mixed solution at a room temperature, performing centrifugal separation on the mixed solution, and performing freeze drying on the obtained solid phase so as to obtain pretreated microalgae powder; (2) performing oil extraction, namely adding the pretreated microalgae powder and an organic solvent into an extracting tank, and heating for extracting; and (3) performing post-treatment, namely cooling the obtained extracting solution to the room temperature after oil extraction, adding deionized water, stirring and standing, finally putting the upper organic phase into a rotary evaporator so as to evaporate the organic solvent, thereby obtaining the microalgal oil. The method for extracting microalgal oil, disclosed by the invention, has the advantages of low cost, high extraction rate, and green environment friendliness.

Description

technical field [0001] The invention relates to the technical field of biodiesel preparation, in particular to a method for extracting microalgae oil. Background technique [0002] Microalgae have the advantages of high photosynthetic efficiency, fast growth rate, strong environmental adaptability and high oil yield, and are the most potential biodiesel raw materials. In the prior art, organic solvent extraction is generally used to extract oil from microalgae. However, most types of microalgae cells have cellulosic cell walls, which are thick and hard, enclosing the microalgae oil in it. If the microalgae oil is directly extracted with traditional organic solvents, the cell wall of the microalgae cells is difficult to break during the extraction process. , resulting in a small amount of microalgae oil released from the cells, resulting in low extraction efficiency. Therefore, in order to further improve the extraction efficiency of microalgae oil by organic solvent extrac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/10C10L1/02
CPCC10L1/02C11B1/04C11B1/108
Inventor 陆伟东王忠铭李少环袁振宏
Owner SHAOGUAN COLLEGE
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