Method for quickly evaluating ecological toxicity of petroleum-contaminated soil

A technology for oil pollution and soil biology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, instruments, etc., can solve the problems of long time, low efficiency, unable to reflect the comprehensive biological toxicity of soil oil pollution, etc.

Inactive Publication Date: 2017-08-25
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have problems such as cumbersome experimental steps, long time consumption, and low efficiency, and the evaluation results can only reflect the toxic effect of petroleum pollutants on the evaluation index organisms, and cannot reflect the comprehensive biological toxicity of soil oil pollution.

Method used

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  • Method for quickly evaluating ecological toxicity of petroleum-contaminated soil
  • Method for quickly evaluating ecological toxicity of petroleum-contaminated soil
  • Method for quickly evaluating ecological toxicity of petroleum-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the selection of induction strain

[0042] Three common luminescent bacteria, Vibrio fischeri, Photobacteriumphosphoreum T3 and Vibrio qinghaiensis Q67 were selected to compare their ability to quickly recover from the freeze-dried state, and their effects on oil pollution According to the concentration sensing range of pollutants and the luminescent response under different environmental factors, luminescent bacteria with strong resurrection ability, high sensitivity and strong stability were selected as soil petroleum pollutant sensing bacteria.

[0043] (1) Resurrection ability of luminescent bacteria

[0044] Compare the time and luminous brightness required for the three kinds of luminescent bacteria to recover from the freeze-dried powder state to normal luminescence (such as figure 1 As shown), it was found that Vibrio fischeri and Photobacterium luminescens T3 had continuous and stable luminescence in 0-20 minutes, and the intensity of luminescenc...

Embodiment 2

[0048] Embodiment 2, the determination of the environmental index of vibrio fischeri

[0049] The environment can affect the growth and metabolic process of luminescent bacteria, thereby affecting their luminous intensity and stability. In order to eliminate the influence of environmental conditions on the luminescence effect of the sensing strains, the luminescence intensity and stability of Vibrio fischeri ( image 3 ). Environmental factors include pH, salinity, temperature.

[0050] It can be seen that Vibrio fischeri has no significant change in the range of pH7-9, and when the pH value is greater than 10, the relative luminescence rate decreases slightly. The relative luminous rate of Vibrio fischeri tends to be stable under the condition of 0-5% salinity, and the relative luminous rate is slightly higher than the average level when the salinity is 2-4%. The luminescence response of Vibrio fischeri was relatively stable at 0-10°C, and when the temperature was higher t...

Embodiment 3

[0052] Embodiment 3, the selection of soil petroleum pollutant extractant

[0053] According to the demand that the extractant not only maintain the activity of luminescent bacteria, but also quickly and efficiently extract petroleum pollutants in the soil, Tween-20 (Tween20), Tween-80 (Tween80), polyethylene glycol octylphenyl ether ( Triton X100), sodium dodecyl sulfate (SDS), sodium dodecylbenzenesulfonate (SDBS) and rhamnolopid (Rhamnolopid) these 6 surfactants are used as alternative extraction agents to select the oil pollutants in the soil extractant.

[0054] The relative luminous rate (%) of Vibrio fischeri in different surfactants of table 1

[0055]

[0056] Comparing the luminous intensity (table 1) of vibrio fischeri in 6 kinds of surfactants with 1% concentration, vibrio fischeri is under Tween20, Tween80, Triton X100, SDBS and Rhamnolopid environment relative luminescence rate is more than 92%, They can survive normally, indicating that these extractants ar...

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Abstract

The invention provides a method for quickly evaluating ecological toxicity of petroleum-contaminated soil. The method comprises the following steps: 1) with rhamnolipid solution as an extracting agent, extracting petroleum pollutants in to-be-detected petroleum-contaminated soil, thereby acquiring a to-be-detected petroleum pollutant extracting solution; 2) mixing the to-be-detected petroleum pollutant extracting solution with luminous bacteria and reacting, and meanwhile, with a NaCl solution as contrast, mixing the NaCl solution with luminous bacteria and reacting for the same time, respectively recording the real-time luminescence volume of the test sample and the contrast and calculating the relative luminous emissivity (%) of the sample; 3) evaluating the ecological toxicity level of the soil sample according to the relative luminous emissivity of the sample. According to the invention, the luminous bacteria are selected as induction bacteria for soil petroleum contamination and a quick detection technology and an evaluation standard for the ecological toxicity of petroleum-contaminated soil are established in the manner of selecting bacteria capable of stably, sensitively and quickly reflecting the change in petroleum contamination concentration in the environment, regarding the light intensity thereof as a detection index and constructing the relation models of light intensity-petroleum contamination concentration and petroleum contamination concentration-toxicity.

Description

technical field [0001] The invention belongs to the field of soil pollution detection, and in particular relates to a method for quickly evaluating the ecological toxicity of oil-polluted soil. Background technique [0002] Petroleum is a complex mixture of various compounds formed by organisms in ancient oceans or lakes through long-term evolution. Petroleum is a black or dark brown viscous liquid at room temperature, with a density of 829-896kg / m 3 , its chemical composition and physical properties vary with the place of origin. The main components of petroleum are hydrocarbons, accounting for more than 95% of the total composition, and the rest are trace amounts of heteroatoms such as nitrogen, oxygen, and sulfur, and trace amounts of metal elements such as vanadium, nickel, and iron. Hydrocarbons can be divided into saturated hydrocarbons (including normal paraffins, isoparaffins and naphthenes), aromatic hydrocarbons (including monocyclic, bicyclic and polycyclic arom...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/763
Inventor 黄艺陈惠苑汪杰曹晓峰许天平黄梵
Owner PEKING UNIV
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