A genetic detection kit for alcohol assessment
A gene detection and kit technology, applied in the field of molecular biology and laboratory medicine, can solve the problems of high sequencing cost, poor research and connection of alcohol consumption, lack of scientific rigor, etc., and achieve intuitive and easy-to-understand test results Effect
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[0031] The technical solutions of the present invention will be described in detail below in conjunction with the drawings and specific embodiments. The specific embodiments described here are only used to explain the present invention, but the protection scope of the present invention is not limited to the embodiments.
[0032] The present invention is based on the RNA level, using the "five-fold" fluorescent quantitative PCR technology to simultaneously detect the expression levels (ΔCt value) of the ADH1B gene and the ALDH2 gene and two SNP mutations whose RS numbers are rs1229984 and rs671. Since the mutations of rs1229984 and rs671 belong to the mutation of the CDS region (protein coding region) of the gene, the SNP site of the mRNA will retain the base type at the DNA level, which is for the simultaneous detection of the internal reference at the RNA level for gene quantification, and ADH1B Gene and ALDH2 gene SNP typing to lay a solid theoretical foundation.
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