Biological activated carbon for degrading sewage COD and preparation method thereof
A biological activated carbon and sewage degradation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems affecting the adsorption performance of macropores and some mesopores in the structure of activated carbon, the growth and reproduction ability of bacteria, and the degradation performance Large, unstable combination of biofilm and activated carbon, etc., to achieve the effect of high market application value, good removal effect, and low production cost
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[0023] A preparation method for bioactive carbon for degrading sewage COD, comprising the following steps:
[0024] 1) Screening, domestication, expanded cultivation and compounding of bacterial strains: select corresponding bacterial strains according to the types and contents of main pollutants in sewage, first carry out domestication and expanded cultivation respectively, and then carry out compounding to obtain compound bacterial strains;
[0025] 2) Pretreatment of activated carbon: Soak the activated carbon with hydrochloric acid for pretreatment, then separate the activated carbon and fully dry it;
[0026] 3) Preparation of biological activated carbon: uniformly mix the pretreated activated carbon and composite strains, culture at a constant temperature of 28-37° C. for 20-30 hours, and fully dry to obtain biological activated carbon.
[0027] Preferably, the medium in step 1) is beef extract liquid medium or beef extract solid medium, pH=7.2-7.5, and the composition o...
Embodiment 1
[0039] The sewage is wastewater from a food processing factory, the COD is nitrate nitrogen, and the selected bacteria are aerobic denitrifying Bacillus, Bacillus subtilis and Bacillus licheniformis.
[0040] 1) Screening, domestication, expanded cultivation and recombination of strains: Sewage or soil samples were collected near the waste water outfall of a food processing factory, and then inoculated on beef extract liquid medium with a potassium nitrate concentration of 500 mg / L. Enrichment culture at 30°C on a shaker for 4 days, and then inoculate the acclimatization culture solution on the beef extract liquid medium with a potassium nitrate concentration of 600mg / L according to the inoculum size of 5%, and enrich and cultivate at 30°C on a shaker for 4 days. Then increase the concentration of potassium nitrate substrate by 100 mg / L each time, and carry out cyclic acclimatization until the concentration of potassium nitrate is 1000 mg / L, then separate the strains in the acc...
Embodiment 2
[0048] The sewage is the wastewater produced by a herbicide factory, the COD is diclopyridine, and the selected bacteria are diclopyridine degrading bacillus, colloid bacillus, subtilis and licheniformis which have the function of diclopyridine degradation.
[0049]1) Screening, acclimatization, expanded cultivation and recombination of strains: collect sewage or soil samples near the wastewater outfall of a herbicide factory, and then inoculate them on beef extract liquid medium with a diclopyridine concentration of 500 mg / L. Enrichment culture on a shaker at 35°C for 3 days, then inoculate the acclimatization culture solution on the beef extract liquid medium with a diclopyridine concentration of 600mg / L according to the inoculum size of 3%, and enrich and culture on a shaker at 30°C for 4 days day, and then increase the concentration of the substrate diclopyridine by 100mg / L each time, and carry out cyclic acclimation until the concentration of diclopyridine is 1000mg / L, the...
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