Composition for regulating enteric microorganisms and preparation method thereof
A technology for regulating intestinal tract and composition, which is applied in the field of medicine, can solve problems such as intestinal microbial flora, unfavorable long-term maintenance, and increase probiotics, etc., to achieve the effect of strengthening killing and cleaning, reducing harmful bacteria, and increasing probiotics
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[0067] The present invention also provides a method for preparing a composition for regulating intestinal microorganisms, comprising the following steps:
[0068] It is obtained by mixing stem cell growth factor, human interferon-λ, human interleukin 2, Artemisia argyi leaf extract, human plasma albumin solution and a solvent.
[0069] The present invention does not limit the source of stem cell growth factor, which can be commercially available, preferably prepared according to the following method:
[0070] The culture supernatant of P3 generation umbilical cord mesenchymal stem cells was collected and concentrated.
[0071] The present invention is not limited to the cultivation and concentration, as long as those skilled in the art are familiar with it.
[0072] The present invention does not limit the source of human interferon-λ, which can be commercially available, preferably prepared according to the following method:
[0073] The culture supernatant of T lymphocytes...
Embodiment 1
[0092] The culture supernatant of P3 generation umbilical cord mesenchymal stem cells is collected and concentrated to obtain stem cell growth factors; the culture supernatant of T lymphocytes is collected and concentrated to obtain cytokines of immune cells. Interferon-λ, Interleukin 2, and Interleukin 11 were purified from immune cytokines; buy Artemisia argyi extract.
[0093] According to the dosage of each component, specifically: stem cell growth factor 50ng / mL, human interferon-λ 500U / mL, human interleukin 2 500U / mL, human interleukin 11 50U / mL, mugwort leaf extract 1μg / mL, 1vol% human plasma albumin solution. Dissolve and dilute the above components in physiological saline under aseptic conditions, so that the final composition volume is 100mL to obtain a composition for regulating intestinal microbes, which is placed in an ampoule bottle, 5mL / bottle.
[0094] The composition described in the present invention is intravenously infused, and the feces of people who have...
Embodiment 2
[0096] The culture supernatant of P3 generation umbilical cord mesenchymal stem cells is collected and concentrated to obtain stem cell growth factors; the culture supernatant of T lymphocytes is collected and concentrated to obtain cytokines of immune cells. Interferon-λ, Interleukin 2, and Interleukin 11 were purified from immune cytokines; buy Artemisia argyi extract.
[0097] According to the dosage of each component, specifically: stem cell growth factor 100ng / mL, human interferon-λ 1000U / mL, human interleukin 2 1000U / mL, human interleukin 11 100U / mL, mugwort leaf extract 2μg / mL, 1vol% human plasma albumin solution. Dissolve and dilute the above components in physiological saline under aseptic conditions, so that the final composition volume is 100mL to obtain a composition for regulating intestinal microbes, which is placed in an ampoule bottle, 5mL / bottle.
[0098] The composition described in the present invention is intravenously infused, and the feces of people who ha...
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