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Pseudomonas nitroreducens as well as fermentation product and application thereof

A technology of Pseudomonas and fermentation products, applied in the field of Pseudomonas nitroreductor and its fermentation products and in the field of cellulose degradation, can solve the problems of severe reaction conditions, long decomposition and transformation cycle, polluted air, etc., to achieve Efficient degradation and high enzyme activity

Inactive Publication Date: 2017-08-04
BEIJING HEHE RUNSHENG TECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] my country is a large agricultural country, and various crop straw resources are very rich, but when the straw is directly returned to the field, it takes a long time to decompose and transform in the soil, so it is difficult to be used as a fertilizer source for seasonal crops
At the same time, about 30% of the remaining crop stalks cannot be effectively processed and utilized every year, and the phenomenon of concentrated burning of stalks during the harvest season has become increasingly prominent, polluting the air and endangering people's health
Using traditional physical and chemical methods, such as acid treatment, alkali treatment, and steam heating, to treat straw and other cellulose, there are problems such as severe reaction conditions, expensive equipment, high cost, and new environmental pollution.

Method used

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  • Pseudomonas nitroreducens as well as fermentation product and application thereof
  • Pseudomonas nitroreducens as well as fermentation product and application thereof
  • Pseudomonas nitroreducens as well as fermentation product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Inoculate the strain GM (that is, the nitroreductor pseudomonas with the preservation number of CGMCC No.13412) onto a GU-PDA plate (glucose: 20 g, potato powder 3 g, potassium dihydrogen phosphate: 3 g, magnesium sulfate: 1.5g, agar: 15g, guaiacol: 1g), in 30 ℃ in constant temperature incubator and cultivate 7d, reddish-brown discoloration circle appears around the bacterium colony, measure the diameter of 7mm and the diameter of discoloration circle ( H) is 13 mm, and the ratio (H / D) is 1.86, indicating that the bacterial strain of the present invention has better ligninase activity.

[0041] (2) Cultivate the strain GM (based on dry weight, the inoculum size is 25mg / L) in a constant temperature (30°C) shaker (170rpm) for 4 days, and the composition of the medium (1L) used is: CMC-Na 10g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 0.3g, peptone 3g, yeast powder 0.5g, (NH 4 ) 2 SO 4 2g, CaCl 2 2H 2 O 0.3g, Tween-80 0.2g, pH 6.5-7; the obtained culture was centrifuged at...

Embodiment 2

[0043] (1) Cultivate bacterial strain GM according to embodiment 1 step (2), the difference is that every 24h is sampled and measured for cellulosic endoenzyme activity and total enzyme activity (that is, filter paper enzyme activity), and making time is the abscissa, Enzyme activity curve with enzyme activity as the coordinate. The relationship between the enzyme activity and the culture time was obtained, and the results were as follows: figure 1 and figure 2 As shown, it can be seen that under liquid culture conditions, the activities of the two enzymes both reached the maximum on the third day, which were 82.35U / ml and 11.87U / ml respectively.

[0044] (2) Cultivate strain GM (based on dry weight, the inoculum size is 25 mg / L) at constant temperature (30°C), and the medium composition used is: 50 g of wheat straw (about 2 mm, taken from Baoding, Hebei), phosphoric acid Potassium dihydrogen 1g, magnesium sulfate heptahydrate 0.025g, ammonium sulfate 1g, deionized water 10...

Embodiment 3

[0046] Dry the 50ml Erlenmeyer flask and the ground wheat straw (about 2mm, taken from Baoding, Hebei) at high temperature and weigh it to the nearest 0.1mg, and record it as W 1 (conical flask) and W 2 (wheat straw). Weigh 1g of wheat straw, 7.5g of inorganic salt solution containing 0.5% by weight of yeast powder (containing 5mg dipotassium hydrogen phosphate, 5mg potassium dihydrogenphosphate, 1mg magnesium sulfate heptahydrate and 5mg ammonium sulfate), and put it into a 50ml Erlenmeyer flask Sterilize at 121°C for 15 minutes. Add 1 ml of strain GM inoculum (2.5 mg based on Pseudomonas nitroreducens), put it in a constant temperature incubator, and incubate at 30°C for 10 days. The strains were used as a parallel control group, and the results were averaged. Sterilized at 121°C for 15 minutes, dried and weighed, recorded as W 3 (total weight of Erlenmeyer flask and culture). According to "degradation rate = (W 1 +W 2 -W 3 ) / W 2 ×100%" to calculate the degradation ...

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Abstract

The invention relates to the field of cellulose degradation and discloses pseudomonas nitroreducens as well as a fermentation product and application thereof. The preservation number of the pseudomonas nitroreducens provided by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.13412. The invention further discloses a method for culturing the pseudomonas nitroreducens; the method comprises the following steps: inoculating the pseudomonas nitroreducens into a fermentation culture medium and fermenting. The invention further discloses a method for degrading cellulose and the method comprises the step of enabling a degradation agent to be in contact with a raw material containing the cellulose; the degradation agent is the pseudomonas nitroreducens and / or the fermentation product of the pseudomonas nitroreducens. Furthermore, the invention discloses application of the pseudomonas nitroreducens and / or the fermentation product of the pseudomonas nitroreducens to degradation of the cellulose. The pseudomonas nitroreducens disclosed by the invention can be used for effectively degrading the cellulose, the fermentation product has various enzyme activities and the enzyme activities are relatively high.

Description

technical field [0001] The invention relates to the field of cellulose degradation, in particular to a strain of Pseudomonas nitroreducens and its fermentation product and its use in degrading cellulose. Background technique [0002] my country is a large agricultural country, and various crop straw resources are very rich. However, when the straw is directly returned to the field, it takes a long time to decompose and transform in the soil, so it is difficult to be used as a fertilizer source for seasonal crops. At the same time, about 30% of the remaining crop stalks cannot be effectively treated and utilized every year, and the phenomenon of concentrated burning of stalks during the harvest season has become increasingly prominent, polluting the air and endangering people's health. Using traditional physical and chemical methods, such as acid treatment, alkali treatment and steam heating, to treat cellulose such as straw, has problems such as severe reaction conditions, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P19/02C12R1/38
CPCC12P1/04C12P19/02C12N1/205C12R2001/38
Inventor 邵阳王兴涛徐粲然任立伟卢滇楠
Owner BEIJING HEHE RUNSHENG TECH CO LTD
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