Method for simultaneously measuring concentrations of six tyrosine kinase inhibitors in blood plasma

Abstract

The invention discloses a method for simultaneously measuring the concentrations of six tyrosine kinase inhibitors in blood plasma. Imatinib is adopted as an internal standard, a medicament in the blood plasma is extracted at first by virtue of a mixed solution of ethyl acetate and tert-butyl methyl ether, then a sample is separated by virtue of high performance liquid chromatography, medicament targeting detection is performed in a high resolution mass spectrometry parallel reaction monitoring mode, and secondary fragment ions of the medicament are used for quantification, so as to realize simultaneous analysis and measurement of the concentrations of the six tyrosine kinase inhibitors in the blood plasma. The method is quick, has the advantages of extremely high targeting performance, high speed, high throughput, high sensitivity, high specificity, high precision and accuracy, high stability, high extraction recovery rate, no obvious substrate effect or dilution effect, and the like, and can be used for plasma concentration monitoring of clinically common antitumor medicaments, i.e. tyrosine kinase inhibitors, and a nanogram-level detection limit can be achieved.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to a method for simultaneously measuring the concentrations of six tyrosine kinase inhibitors in blood plasma. Background technique [0002] For a long time, cancer has attracted much attention because of its high mortality rate. Current studies have found that cancer is a disease induced by multiple factors, including genome disorder, irregular diet, environmental pollution, radiation and so on. Compared with normal cells, cancer cells have a stronger ability to replicate to resist apoptosis. Clinically, surgery, radiotherapy, chemical drug therapy and targeted drug therapy are mainly used for treatment, among which postoperative chemical drug therapy is one of the commonly used methods. Most chemotherapeutic drugs have poor specificity and usually lead to severe toxic side effects. Therefore, targeted drugs with high specificity are gradually entering the clinic. [0003...

AI Technical Summary

Problems solved by technology

These methods still need to be improved in terms of sensitivity, specificity and high throughput

In addition, the detection standards of these methods are not uniform

Images