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A high-throughput method for SSR molecular markers related to plant organ development

A molecular marker and plant organ technology, applied in the field of molecular biology, can solve the problems of low development efficiency and high price of SSR molecular markers for Chinese rose, and achieve the effect of huge quantity, large quantity and good polymorphism.

Active Publication Date: 2020-07-17
CHINA AGRI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem of low efficiency and high price in the development of rose SSR molecular markers in the prior art, the present invention proposes a high-throughput method for SSR molecular markers related to plant organ development

Method used

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  • A high-throughput method for SSR molecular markers related to plant organ development
  • A high-throughput method for SSR molecular markers related to plant organ development
  • A high-throughput method for SSR molecular markers related to plant organ development

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Experimental program
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Effect test

Embodiment 1

[0037] The method for SSR molecular markers related to the development of salmansa rose flower organs comprises the following steps:

[0038] (1) Sequencing the genome and flower organ transcriptome of the commonly planted rose variety 'Samantha' (samantha) by next-generation sequencing, obtained 32G and 7.2G of raw data respectively, after quality control (only high In Q30 (sequencing data with an accuracy greater than 99.9%) and de novo assembly (using SOAPdenovo and Trinity software to assemble rose genome and floral organ transcriptome respectively) about 3 million genome fragment sequences and 8 10,000 transcript sequences;

[0039] (2) Through the software MISA ( http: / / pgrc.ipk-gatersleben.de / misa / ) identify the microsatellite sequences (simple sequence repeat, SSR) existing in the genome fragment sequence and the transcript sequence in step (1), and design primers at both ends of these microsatellite sequences, and the annealing temperature of the primers is required ...

Embodiment 2

[0045] (1) Extraction of genomic DNA

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Abstract

The invention discloses a high-throughput plant organ development related SSR molecular marker method of the molecular biology field. According to the method provided by the invention, with the flowers of rose as the example, more than hundred thousand of SSR primers can be obtained at one time, and 3387 pairs of the SSR primers are related to functional genes expressed in a rose development process. A lot of SSR molecular markers are selected, have polymorphism, are suitable for different application selections, can well provide genetic background for analysis and excavation of related genes, understand the domestication history of Chinese old rose, cultivation domestication of rose, and rose germplasm resource diversity, and also can provide a set of SSR molecular marker system for relative relationship identification and genetic map construction of other rosaceae plants. The method provided by the invention almost completely relies on computer simulation and calculation, gets rid of the condition restriction of laboratories and the influence of uncontrollable factors, and the obtained molecular markers are large in quantity, reliable, and have good polymorphism.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a high-throughput SSR molecular marker method related to plant organ development. Background technique [0002] Rose belongs to Rosaceae Rosaceae plant, is the largest cut flower in the world, and has high ornamental value. After long-term natural selection and complex cultivation and domestication, roses have evolved from wild species to ancient roses, and then from ancient roses to modern roses. Since the creation of the first modern rose "France" in 1867, there have been about 33,000 modern rose cultivars. Modern roses are generally tetraploid, and most of them are new varieties bred by repeated crosses between Chinese roses and other original European rose varieties. Therefore, the study of genetic diversity and population structure is an important content of rose biology research, and it is the basis for the discovery of genes of important traits of rose and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895G16B30/20G16B30/00
CPCC12Q1/6895C12Q2600/156G16B30/00
Inventor 张钊戚维聪方培红曹晓倩刘欣童石少川陈曦
Owner CHINA AGRI UNIV
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