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Strain of Metarhizium anisopliae FM-03 and its application in prevention and control of mealybugs

A technology of FM-03, Metarhizium anisopliae, applied in application, fungi, microorganism-based methods, etc., to achieve the effect of maintaining ecological balance, high spore germination rate, and large spore production

Active Publication Date: 2017-07-28
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Metarhizium anisopliae ( Metarhizium spp.) is an entomogenous fungus with a wide geographical distribution and host range. At present, some strains have been screened for the control of mealybugs, such as Liang Changcong et al. Brontispa longissina ) isolated on the pineapple mealybug ( Dysmicoccus brevipes ) Metarhizium anisopliae with strong lethality ( Metarhizium sp.) J813 strain (Anhui Agricultural Science, CN: 34-1076 / S), Huang Junsheng et al. (2012) reported that the sugarcane borer ( Chilo venosatus ) and mealybug cinerea ( Dysmicoccus neobrevipes ) and other pests with strong pathogenicity Metarhizium anisopliae ( Metarhizium anisopliae ) JF-6213 strain (ZL 2012 10274297.1), but these strains are only a small part of the Metarhizium anisopliae strains, and there are many efficient strains from other hosts and geographical sources to be discovered

Method used

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  • Strain of Metarhizium anisopliae FM-03 and its application in prevention and control of mealybugs
  • Strain of Metarhizium anisopliae FM-03 and its application in prevention and control of mealybugs

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Effect test

Embodiment 1

[0013] Metarhizium anisopliae ( Metarhizium anisopliae ) Isolation and identification of FM-03 and determination of spore production and spore germination rate

[0014] 1. Test method

[0015] 1) Isolation of strains: Collected from the compost near the Wusi Youth Farm in Yanxi Town, Changtai County, Zhangzhou, Fujian, the dark gill beetle ( Holotrichia parallela ) larvae, use a sterilized scalpel to scrape the spore powder on the surface of the worm body into 10 mL of sterile water, filter the spore stock solution through degreasing gauze, and then spread the spore stock solution evenly on the PDA plate medium, and then place it at a temperature (25 ±1)°C, relative humidity (90±5)%, and photoperiod L:D=12:12 in a light incubator for 8 days.

[0016] 2) Morphological identification: After the isolated strain was cultured for 8 days, first observe the characteristics of the colony and conidia, and then use an inverted microscope to observe the mycelium width, conidia shape ...

Embodiment 2

[0026] Metarhizium anisopliae ( Metarhizium anisopliae ) FM-03 against citrus mealybug (P. Pseudococcus longispinus ), Mealyaccus custard apple (P. Planococcus lilacinus ) and succulent mealybugs (P. Phenacoccus solani ) and other mealybug infection lethal effects

[0027] 1, test method: get (embodiment 1) isolated metarhizium anisopliae ( Metarhizium anisopliae ) FM-03, inoculated on PDA plate medium, placed in a light incubator at temperature (25±1)°C, relative humidity (90±5)%, and photoperiod L:D=12:12 for 8 days; Add 10 mL of sterile water containing 0.05% Tween-80 to the petri dish to delyze the spores, filter through three layers of degreasing gauze to obtain the spore stock solution; use a Neubauer hemocytometer to count the number of spores in the spore stock solution under a biological microscope and count the spores Concentration, and then dilute the spore stock solution with sterile water to 1.00×10 4 , 1.00×10 5 , 1.00×10 6 , 1.00×10 7 and 1.00×10 ...

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Abstract

The invention relates to a strain of Metarhizium anisopliae FM-03 and its application in prevention and control of mealybugs and belongs to the technical field of biological prevention and control. The Metarhizium anisopliae FM-03 has an accession number of CGMCC No. 13774. The growth performance of Metarhizium anisopliae is good, the sporulation rate is high, the germination rate of spores is high, and the lethal effect on mealybugs is good. The Metarhizium anisopliae FM-03 is safe to the natural enemy, has the potential to replace a part of the mealybug chemical pesticide, reduces a use amount of a chemical pesticide and is conducive to keep the ecological balance.

Description

technical field [0001] The invention relates to a metarhizium anisopliae FM-03 and its application in preventing and treating mealybug, belonging to the technical field of biological control. Background technique [0002] Pseudococcus is a pest of Hemiptera (Pseudococcidae) with a wide range of hosts. It is an important pest of tropical and subtropical economic crops and temperate greenhouse plants. It causes leaf fall, flower drop and fruit drop. The honeydew secreted can induce soot Diseases aggravate the damage, directly or indirectly affect the yield, quality and economic value of crops, and are not easy to control. At present, the prevention and control of this pest focuses on the use of chemical pesticides, which can easily cause "3R" and phytotoxicity problems. [0003] Biological control is a safe, effective and long-lasting pest control method, and the excavation and utilization of entomogenic fungi has become one of the important development directions of pest bio...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 黄鹏余德亿姚锦爱蓝炎阳
Owner INST OF PLANT PROTECTION FAAS
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