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Microbial preparation for treating excrement, preparation method thereof and application

A microbial preparation and microbial technology, applied in the field of animal husbandry, can solve the problems of large production volume and difficult treatment, and achieve the effect of solving technical problems and solving heavy pollution control

Inactive Publication Date: 2017-07-25
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pollutants in animal husbandry farms have a high concentration of organic matter and odorous gases. At present, some new treatment processes have emerged to solve the problems of large production volume and difficult treatment.

Method used

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  • Microbial preparation for treating excrement, preparation method thereof and application
  • Microbial preparation for treating excrement, preparation method thereof and application
  • Microbial preparation for treating excrement, preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the enrichment of high-efficiency microbial preparation

[0032] Basic liquid medium (W / V) contains 1.0% rice bran, 1.0% rapeseed meal, 2.0% fish meal, 0.5% rice straw; weigh 0.1g indole and skatole (3-methylindole), dissolve in 1mL anhydrous Add 100 μL (0.1%, V / V) acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isovaleric acid and 100 μL (0.01% , W / V) indole, skatole (3-methylindole). In order to mix well, indole and skatole can be dissolved in absolute ethanol first, and then added to the medium. Adjust the pH to 6.0, and sterilize with high pressure steam at 121°C for 20 minutes.

[0033] Weigh 0.1g of composted cow dung, inoculate it in the above liquid medium, inoculum size 0.1% (W / V), 30°C, 200r min -1 Shaking table culture; continuous culture for 48 hours, the collected bacterial liquid is used as seed culture liquid for solid enrichment and scale-up cultivation.

Embodiment 2

[0034] Embodiment 2: the expansion cultivation of high-efficiency microbial preparation (1 kilogram)

[0035] The solid culture substrate contains (W / W) 38.0% rice bran, 10.0% rape meal, 10.0% fish meal, 14.0% straw, 3.0% cow dung, and the rest is water. Put the prepared medium in a large glass beaker, cover it with aluminum foil, put it in a sterilizer, and sterilize it by high-pressure steam at 121°C for 20 minutes;

[0036] Get 50g of the seed culture solution enriched in Example 1, insert it into the above-mentioned solid culture medium cooled to room temperature after sterilization, the inoculum size is 5.0% (W / W), and add 0.1% ( V / V) acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isovaleric acid and 0.02% (W / V) of indole and skatole. In order to mix well, indole and skatole can be dissolved in absolute ethanol first, and then added to the medium. After fully mixing evenly, put the mixture into a 1kg breathing bag, seal it, put it in a con...

Embodiment 3

[0038] Embodiment 3: the expansion cultivation of high-efficiency microbial preparation (10 kilograms)

[0039]First prepare solid culture medium 1kg, the formula of 1kg medium is as follows (W / W): 38.0% rice bran, 10.0% rape meal, 10.0% fishmeal, 14.0% rice straw, 3.0% cow dung, all the other are moisture, culture medium is put in large in a non-woven bag, placed in a steel tray, placed in a sterilizer, and sterilized by high-pressure steam at 121°C for 20 minutes;

[0040] Get 500g of the solid culture after expansion in Example 2, insert it into the above-mentioned solid culture medium, inoculum size 5.0% (W / W), mix evenly, pack into a 10kg breathing bag, and add 0.1 to the above-mentioned mixture respectively % (V / V) of acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, isovaleric acid and 0.01% (W / V) of indole and skatole. In order to mix well, indole and skatole can be dissolved in absolute ethanol first, and then added to the medium. Seal th...

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Abstract

The invention discloses a microbial preparation for treating excrement, a preparation method thereof and an application. The microbial preparation is acquired through adding organic acid and excrement compound in a culture medium and then performing microbial gathering and domestication; the viable bacterial colony number in the microbial preparation is 5x108-2x109 CFU / g. The added organic acid includes acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-pentanoic acid and isovaleric acid; the excrement compound comprises benzpyrole and skatole. The microbial preparation prepared by the invention can be applied to the in-situ harmless treatment of fecaluria at cow breeding bed; through degrading cellulose, hemicellulose, organic amino nitrogen and phosphorus, organic acid, and other excrement components, the microbial preparation reaches the technical effects of achieving zero emission and pollution cleaning, inhibiting harmful bacterium multiplication, and improving the cow feeding environment, and others.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a microbial preparation for treating feces, a preparation method and application thereof. Background technique [0002] Animal husbandry is an important part of agriculture. Since the mid-1990s, my country's animal husbandry has gradually formed a management mode of centralized breeding and large-scale production, but the ensuing environmental pollution and manure discharge have gradually become social concerns. One of the hotspots, with the promulgation and strict implementation of various pollution-related regulations, many enterprises that do not meet the environmental discharge standards have to face the dilemma of shutting down. [0003] Pollutants in animal husbandry farms have high concentrations of organic matter and malodorous gases. At present, some new treatment processes have emerged to solve the problems of large production volume and difficult tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C02F11/02C12R1/07C12R1/01C12R1/66
Inventor 孙俊松赵利超吴海英史吉平姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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